Detailed information of TA system TA033240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2726458..2726987
• Replicon: chromosome
• Accession: NZ_AP020311
• Organism: Staphylococcus aureus strain KUH140013

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F6Y13_RS13795
• Protein ID: WP_000621175.1
• Coordinates: 2726625..2726987 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: F6Y13_RS13790
• Protein ID: WP_000948331.1
• Coordinates: 2726458..2726628 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y13_RS13760	2721494..2722054	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
F6Y13_RS13765	2722263..2722742	+	480	WP_001287088.1	hypothetical protein	-
F6Y13_RS13770	2722735..2724318	+	1584	WP_001294626.1	PH domain-containing protein	-
F6Y13_RS13775	2724305..2724796	+	492	WP_001205910.1	PH domain-containing protein	-
F6Y13_RS13780	2724800..2725159	+	360	WP_000581200.1	holo-ACP synthase	-
F6Y13_RS13785	2725225..2726373	+	1149	WP_001281145.1	alanine racemase	-
F6Y13_RS13790	2726458..2726628	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
F6Y13_RS13795	2726625..2726987	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6Y13_RS13800	2727337..2728338	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
F6Y13_RS13805	2728457..2728783	+	327	WP_001052491.1	anti-sigma factor antagonist	-
F6Y13_RS13810	2728785..2729264	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
F6Y13_RS13815	2729239..2730009	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T33240 WP_000621175.1 NZ_AP020311:2726625-2726987 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T33240 NZ_AP020311:2726625-2726987 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT33240 WP_000948331.1 NZ_AP020311:2726458-2726628 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT33240 NZ_AP020311:2726458-2726628 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1