Detailed information of TA system TA033239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2706716..2707230
• Replicon: chromosome
• Accession: NZ_AP020311
• Organism: Staphylococcus aureus strain KUH140013

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: F6Y13_RS13705
• Protein ID: WP_000581792.1
• Coordinates: 2707021..2707230 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: F6Y13_RS13700
• Protein ID: WP_000138932.1
• Coordinates: 2706716..2707009 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y13_RS13665	2701979..2702620	+	642	WP_000483153.1	thiamine phosphate synthase	-
F6Y13_RS13670	2702706..2703578	+	873	WP_000725802.1	membrane protein insertase YidC	-
F6Y13_RS13690	2704391..2705038	-	648	WP_001187629.1	HD domain-containing protein	-
F6Y13_RS13695	2705066..2706550	-	1485	WP_000571549.1	cardiolipin synthase	-
F6Y13_RS13700	2706716..2707009	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
F6Y13_RS13705	2707021..2707230	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
F6Y13_RS13710	2707358..2707495	+	138	WP_000828354.1	hypothetical protein	-
F6Y13_RS13715	2707759..2708961	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
F6Y13_RS13720	2709279..2710349	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
F6Y13_RS13725	2710364..2711722	+	1359	WP_000611465.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T33239 WP_000581792.1 NZ_AP020311:2707021-2707230 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T33239 NZ_AP020311:2707021-2707230 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT33239 WP_000138932.1 NZ_AP020311:2706716-2707009 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT33239 NZ_AP020311:2706716-2707009 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13