Detailed information of TA system TA033221

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 1447034..1448466
• Replicon: chromosome
• Accession: NZ_AP020310
• Organism: Streptococcus agalactiae strain GCMC97051

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: A0A126UMF2
• Locus tag: SAGCMC97051_RS07565
• Protein ID: WP_024383472.1
• Coordinates: 1447621..1448466 (+)
• Length: 282 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: -
• Locus tag: SAGCMC97051_RS07560
• Protein ID: WP_024383473.1
• Coordinates: 1447034..1447624 (+)
• Length: 197 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAGCMC97051_RS07560 (SAGCMC97051_14190)	1447034..1447624	+	591	WP_024383473.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
SAGCMC97051_RS07565 (SAGCMC97051_14200)	1447621..1448466	+	846	WP_024383472.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
SAGCMC97051_RS07570 (SAGCMC97051_14210)	1448528..1451329	-	2802	WP_065733382.1	phage tail tip lysozyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(O)	hylB / wbtL / lmb / scpB	1332114..1482039	149925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-258)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-50)

Sequences

Toxin

Protein

• Length: 282 a.a.
• Molecular weight: 32435.11 Da
• Isoelectric Point: 5.7531

>T33221 WP_024383472.1 NZ_AP020310:1447621-1448466 [Streptococcus agalactiae]
MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSTYSNHYIFKGGFLLSNVIGVESRSTVDIDFLFHQITLSED
TVKQQLKEILADSKEGISFTIQSITAIKESDDYGGYRATILCQIENIKQVIHLDIATGDVVTPQPITYDYKAIFDEDNFP
IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKEDIDFIQLKNACQRTFTYRETELDFAKIIELLERFKSDP
TQNQQWQNYSKKYSYTKGISLADVLDEMIRLITVLISINSD

Nucleotide

• Length: 846 bp

>T33221 NZ_AP020310:1447621-1448466 [Streptococcus agalactiae]
ATGAATAAAGCTAAGCTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGATTAACCTTTAATTCCGTGATGACCTA
TTACTTTCTTGAAGTGATTTTGAAAAAATTAAGTCAGAGTACCTATTCAAATCACTATATATTCAAAGGAGGATTCCTAC
TGTCAAATGTTATCGGAGTTGAATCTCGTAGCACGGTTGATATTGATTTTTTGTTCCATCAAATAACACTTTCAGAAGAT
ACTGTGAAGCAGCAACTCAAGGAAATTTTAGCAGATTCAAAAGAAGGTATTTCTTTCACAATTCAATCTATCACAGCAAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACCATTTTGTGCCAAATTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAGAAACTTCAAACCATCTATTCACGCAACTTCTTGAACAGTAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCAAAACTGAAGAAAGAAGACATTGACTTCATTCAGTTGAAAAATGCCTGTC
AGAGAACATTTACATATCGCGAAACAGAACTCGATTTTGCAAAGATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAAGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTCGTCTTATAACAGTACTCATTTCTATAAATTCTGATTAA

Antitoxin

Protein

• Length: 197 a.a.
• Molecular weight: 23251.85 Da
• Isoelectric Point: 7.8476

>AT33221 WP_024383473.1 NZ_AP020310:1447034-1447624 [Streptococcus agalactiae]
MSKKEILLEFIENHNGIITYKDCKALKIPTIYLTRLEKEGILYRVEKGIFLTQNGDYDEYYFFQYRYPKAIFSYISALYL
QQFTDEIPQYFDVTIPRGYRFNTPPANLNIHSVSKEYSELGITLVKTPMGNEVKVYDLERIICDFVIHREKIDTELFVKT
LQHYGNYSKKNLTKLYEYATKMNTLDKVKQTLEVLV

Nucleotide

• Length: 591 bp

>AT33221 NZ_AP020310:1447034-1447624 [Streptococcus agalactiae]
ATGTCAAAAAAAGAGATTCTACTTGAATTTATAGAAAACCACAATGGCATTATCACCTATAAAGATTGTAAAGCTCTAAA
GATTCCAACAATATATTTGACAAGACTAGAAAAAGAAGGGATTCTATACCGCGTCGAAAAAGGAATTTTCCTAACCCAGA
ATGGAGACTACGACGAATACTACTTCTTTCAGTACCGTTATCCCAAGGCTATTTTCTCTTATATCTCAGCGCTTTACTTG
CAACAATTTACAGATGAAATTCCTCAATATTTTGACGTAACCATTCCTAGAGGCTACCGTTTTAATACTCCTCCAGCAAA
TCTGAACATTCATTCCGTTTCTAAGGAATATAGCGAACTAGGAATTACACTTGTAAAAACCCCTATGGGAAACGAGGTAA
AAGTGTATGATTTAGAACGTATTATCTGTGATTTTGTGATTCATCGAGAAAAAATAGATACTGAGCTTTTTGTCAAAACG
CTTCAACATTACGGAAACTATTCTAAAAAAAATCTTACAAAACTCTATGAATATGCGACAAAAATGAACACTTTGGACAA
GGTCAAACAAACTCTGGAGGTCCTAGTATGA

Structures

Toxin

• AlphaFold DB: A0A126UMF2