Detailed information of TA system TA033219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 1411778..1413042
• Replicon: chromosome
• Accession: NZ_AP020310
• Organism: Streptococcus agalactiae strain GCMC97051

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: -
• Locus tag: SAGCMC97051_RS07370
• Protein ID: WP_024383486.1
• Coordinates: 1411778..1412566 (-)
• Length: 263 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: SAGCMC97051_RS07375
• Protein ID: WP_024383485.1
• Coordinates: 1412566..1413042 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAGCMC97051_RS07340 (SAGCMC97051_13790)	1406843..1407208	-	366	WP_000431640.1	MobC family plasmid mobilization relaxosome protein	-
SAGCMC97051_RS07345 (SAGCMC97051_13800)	1407211..1407573	-	363	WP_000140674.1	SAG1252 family conjugative relaxosome accessory protein	-
SAGCMC97051_RS07350 (SAGCMC97051_13820)	1407911..1408087	-	177	WP_001873137.1	hypothetical protein	-
SAGCMC97051_RS07355 (SAGCMC97051_13830)	1408093..1408503	-	411	WP_236712219.1	hypothetical protein	-
SAGCMC97051_RS07360 (SAGCMC97051_13840)	1408500..1410041	-	1542	WP_024383488.1	UvrD-helicase domain-containing protein	-
SAGCMC97051_RS07365 (SAGCMC97051_13850)	1410034..1411788	-	1755	WP_024383487.1	AAA family ATPase	-
SAGCMC97051_RS07370 (SAGCMC97051_13860)	1411778..1412566	-	789	WP_024383486.1	type II toxin-antitoxin system toxin PezT	Toxin
SAGCMC97051_RS07375 (SAGCMC97051_13870)	1412566..1413042	-	477	WP_024383485.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
SAGCMC97051_RS07380 (SAGCMC97051_13880)	1413112..1413402	-	291	WP_000667951.1	hypothetical protein	-
SAGCMC97051_RS07385 (SAGCMC97051_13890)	1413452..1413841	-	390	WP_044475264.1	DUF5945 family protein	-
SAGCMC97051_RS07390 (SAGCMC97051_13900)	1413838..1414065	-	228	WP_000110922.1	DUF5965 family protein	-
SAGCMC97051_RS07395 (SAGCMC97051_13910)	1414093..1415193	-	1101	WP_237395018.1	toprim domain-containing protein	-
SAGCMC97051_RS07400 (SAGCMC97051_13920)	1415233..1415871	-	639	WP_060458061.1	hypothetical protein	-
SAGCMC97051_RS07405	1415967..1416257	-	291	WP_024408865.1	DUF5966 family protein	-
SAGCMC97051_RS07410 (SAGCMC97051_13930)	1416271..1416570	-	300	WP_024408864.1	DUF5962 family protein	-
SAGCMC97051_RS07415 (SAGCMC97051_13940)	1416641..1417600	-	960	Protein_1388	hypothetical protein	-
SAGCMC97051_RS07420	1417593..1417914	-	322	Protein_1389	DUF4368 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(O)	hylB / wbtL / lmb / scpB	1332114..1482039	149925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 263 a.a.
• Molecular weight: 30010.08 Da
• Isoelectric Point: 6.6486

>T33219 WP_024383486.1 NZ_AP020310:c1412566-1411778 [Streptococcus agalactiae]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDRPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDRIVENIVDNLRQLEREKLFDCIQIYQRDRTCIYDSETDEGSAAEVLQECLFGKWSKVEEEMM
KFGQERLVKLNNKNLLESNYGI

Nucleotide

• Length: 789 bp

>T33219 NZ_AP020310:c1412566-1411778 [Streptococcus agalactiae]
ATGAGGTTGGAAGAATTTAGTGAAGCTGAGTTTCAGAAGGCTTTACAGCGAACCATTCGGGCTTTAACCCGAGGAAAGAC
GATCCCAGATCGACCAAAAGCTATCTTGCTTGGAGGACAAAGCGGGGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AAGAATTTCAAGGTAATATCATTATCATCGATGGTGATAGCTACCGTTCTCAGCATCCCAATTACTTAGCCTTGCAAGAA
AAGTATGGCAAGGACAGCGTGGACTATACTAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAGGGTTATCACTTGCTGATTGAGGGAACCTTACGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCGA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGCACTAAACCAGAACTGTCATATCTCAGCACCTTAATTCGTTACGAGGAA
CTTTATGCCATAGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCATGATAGGATTGTAGAGAACATAGTTGATAA
CCTAAGGCAGTTAGAAAGAGAGAAACTCTTTGATTGTATTCAGATTTATCAAAGAGACAGGACTTGCATTTATGATTCTG
AAACTGATGAAGGCTCCGCAGCAGAAGTGTTACAAGAGTGTCTCTTTGGAAAATGGAGTAAGGTAGAGGAAGAGATGATG
AAGTTTGGACAGGAACGGTTAGTTAAACTGAATAATAAGAATTTGCTGGAGAGTAACTATGGAATTTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18287.70 Da
• Isoelectric Point: 4.4042

>AT33219 WP_024383485.1 NZ_AP020310:c1413042-1412566 [Streptococcus agalactiae]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYQLTLKVE
VIKERGAAILSQLYRYQDSQDIAFDDESNPWILMSDDLAELINTKIYLVDTFDEIERYNGYLDGIERMLEMVHHRVVA

Nucleotide

• Length: 477 bp

>AT33219 NZ_AP020310:c1413042-1412566 [Streptococcus agalactiae]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCAACTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCACAGCTTTATAGATACCAAGATAGTCAAGATATTGCTTTTGATGATGA
ATCCAACCCTTGGATTTTGATGAGTGATGATTTGGCTGAATTGATCAATACAAAGATTTACCTTGTCGATACTTTCGATG
AGATTGAACGTTACAATGGTTATTTAGACGGTATTGAGCGGATGTTAGAGATGGTGCATCATCGGGTGGTGGCTTAA