Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4561535..4562130 Replicon chromosome
Accession NZ_AP019856
Organism Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag EcoO8H8_RS21670 Protein ID WP_000239579.1
Coordinates 4561780..4562130 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A240D6C8
Locus tag EcoO8H8_RS21665 Protein ID WP_001223212.1
Coordinates 4561535..4561786 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoO8H8_RS21655 4557200..4560979 + 3780 WP_001462977.1 autotransporter assembly complex protein TamB -
EcoO8H8_RS21660 4560982..4561323 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EcoO8H8_RS21665 4561535..4561786 + 252 WP_001223212.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EcoO8H8_RS21670 4561780..4562130 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
EcoO8H8_RS21675 4562210..4562740 - 531 WP_000055072.1 inorganic diphosphatase -
EcoO8H8_RS21680 4563050..4564006 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EcoO8H8_RS21685 4564316..4565818 + 1503 WP_153625572.1 sugar ABC transporter ATP-binding protein -
EcoO8H8_RS21690 4565832..4566854 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T33208 WP_000239579.1 NZ_AP019856:4561780-4562130 [Escherichia coli O8:H8]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T33208 NZ_AP019856:4561780-4562130 [Escherichia coli O8:H8]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9301.61 Da        Isoelectric Point: 4.6509

>AT33208 WP_001223212.1 NZ_AP019856:4561535-4561786 [Escherichia coli O8:H8]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT33208 NZ_AP019856:4561535-4561786 [Escherichia coli O8:H8]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A240D6C8

References