Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3097728..3098382 Replicon chromosome
Accession NZ_AP019856
Organism Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EcoO8H8_RS14725 Protein ID WP_000244772.1
Coordinates 3097728..3098135 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EcoO8H8_RS14730 Protein ID WP_000354046.1
Coordinates 3098116..3098382 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoO8H8_RS14705 3093685..3095418 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
EcoO8H8_RS14710 3095424..3096134 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EcoO8H8_RS14715 3096159..3097055 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EcoO8H8_RS14720 3097167..3097688 + 522 WP_001055874.1 flavodoxin FldB -
EcoO8H8_RS14725 3097728..3098135 - 408 WP_000244772.1 protein YgfX Toxin
EcoO8H8_RS14730 3098116..3098382 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EcoO8H8_RS14735 3098625..3099605 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EcoO8H8_RS14740 3099801..3100460 - 660 WP_000250274.1 hemolysin III family protein -
EcoO8H8_RS14745 3100624..3100935 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EcoO8H8_RS14750 3100980..3102413 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
EcoO8H8_RS14755 3102470..3103213 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T33203 WP_000244772.1 NZ_AP019856:c3098135-3097728 [Escherichia coli O8:H8]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T33203 NZ_AP019856:c3098135-3097728 [Escherichia coli O8:H8]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT33203 WP_000354046.1 NZ_AP019856:c3098382-3098116 [Escherichia coli O8:H8]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT33203 NZ_AP019856:c3098382-3098116 [Escherichia coli O8:H8]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References