Detailed information of TA system TA033200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2638876..2639501
• Replicon: chromosome
• Accession: NZ_AP019856
• Organism: Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EcoO8H8_RS12490
• Protein ID: WP_000911330.1
• Coordinates: 2638876..2639274 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EcoO8H8_RS12495
• Protein ID: WP_000450524.1
• Coordinates: 2639274..2639501 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoO8H8_RS12470	2634754..2634954	+	201	WP_000383836.1	YpfN family protein	-
EcoO8H8_RS12475	2635064..2635762	-	699	WP_000679812.1	esterase	-
EcoO8H8_RS12480	2635836..2637851	-	2016	WP_096306925.1	tRNA cytosine(34) acetyltransferase TmcA	-
EcoO8H8_RS12485	2637866..2638729	-	864	WP_001267495.1	neutral zinc metallopeptidase	-
EcoO8H8_RS12490	2638876..2639274	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EcoO8H8_RS12495	2639274..2639501	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EcoO8H8_RS12500	2639655..2640368	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EcoO8H8_RS12505	2640581..2641615	-	1035	WP_001491244.1	outer membrane protein assembly factor BamC	-
EcoO8H8_RS12510	2641632..2642510	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EcoO8H8_RS12515	2642656..2643228	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EcoO8H8_RS12520	2643228..2643698	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T33200 WP_000911330.1 NZ_AP019856:c2639274-2638876 [Escherichia coli O8:H8]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T33200 NZ_AP019856:c2639274-2638876 [Escherichia coli O8:H8]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT33200 WP_000450524.1 NZ_AP019856:c2639501-2639274 [Escherichia coli O8:H8]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT33200 NZ_AP019856:c2639501-2639274 [Escherichia coli O8:H8]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33