33189

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 495808..496426 Replicon chromosome
Accession NZ_AP019856
Organism Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EcoO8H8_RS02230 Protein ID WP_001291435.1
Coordinates 495808..496026 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EcoO8H8_RS02235 Protein ID WP_000344800.1
Coordinates 496052..496426 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoO8H8_RS02195 491097..491669 + 573 WP_000779831.1 YbaY family lipoprotein -
EcoO8H8_RS02200 491700..492011 - 312 WP_000409911.1 MGMT family protein -
EcoO8H8_RS02210 492389..492742 + 354 WP_096306792.1 DUF1428 family protein -
EcoO8H8_RS02215 492784..494334 - 1551 WP_001503909.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EcoO8H8_RS02220 494498..494968 - 471 WP_000136192.1 YlaC family protein -
EcoO8H8_RS02225 495084..495635 - 552 WP_000102564.1 maltose O-acetyltransferase -
EcoO8H8_RS02230 495808..496026 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EcoO8H8_RS02235 496052..496426 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EcoO8H8_RS02240 496972..500121 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EcoO8H8_RS02245 500144..501337 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33189 WP_001291435.1 NZ_AP019856:c496026-495808 [Escherichia coli O8:H8]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T33189 NZ_AP019856:c496026-495808 [Escherichia coli O8:H8]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT33189 WP_000344800.1 NZ_AP019856:c496426-496052 [Escherichia coli O8:H8]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT33189 NZ_AP019856:c496426-496052 [Escherichia coli O8:H8]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References