Detailed information of TA system TA033186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 12030..13681
• Replicon: plasmid pConjugative
• Accession: NZ_AP019854
• Organism: Neisseria gonorrhoeae strain SS3160

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: -
• Locus tag: FYK59_RS11690
• Protein ID: WP_033911112.1
• Coordinates: 12476..13681 (-)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: FYK59_RS11685
• Protein ID: WP_003701903.1
• Coordinates: 12030..12461 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK59_RS11655	7140..7916	+	777	WP_003701891.1	P-type conjugative transfer protein TrbJ	-
FYK59_RS11660	8130..9757	+	1628	Protein_9	P-type conjugative transfer protein TrbL	-
FYK59_RS11665	9744..10322	+	579	WP_033911116.1	TrbM protein	-
FYK59_RS11670	10322..10921	+	600	WP_003701898.1	TrbN protein	-
FYK59_RS11675	11024..11314	+	291	WP_003701899.1	virulence factor	-
FYK59_RS11680	11424..11978	-	555	WP_003701901.1	recombinase family protein	-
FYK59_RS11685	12030..12461	-	432	WP_003701903.1	hypothetical protein	Antitoxin
FYK59_RS11690	12476..13681	-	1206	WP_033911112.1	zeta toxin family protein	Toxin
FYK59_RS11695	13688..13873	-	186	WP_014580058.1	hypothetical protein	-
FYK59_RS11700	13907..15115	-	1209	WP_014580057.1	zeta toxin family protein	-
FYK59_RS11705	15112..15360	-	249	WP_014580056.1	hypothetical protein	-
FYK59_RS11710	15830..16318	+	489	WP_014580055.1	hypothetical protein	-
FYK59_RS11715	16470..17366	-	897	WP_003701917.1	hypothetical protein	-
FYK59_RS11720	17370..17741	-	372	WP_003701919.1	hypothetical protein	-
FYK59_RS11725	17725..18093	-	369	WP_003701920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39057	39057

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44747.59 Da
• Isoelectric Point: 7.2930

>T33186 WP_033911112.1 NZ_AP019854:c13681-12476 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGREASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T33186 NZ_AP019854:c13681-12476 [Neisseria gonorrhoeae]
ATGGTAAAACTGTCTAGCGATATTAATCTGAGAGATTTTGGCAATAACGAATATTTGTCTTCCGTTCAGGATGAGGCCAT
CCGTTTTGCGACCGAACAAACAGACGAGATATTGTCTTTATATAGCCAACATGCAGATACAGAAGGCGGTAGATATGTTT
GTGCTGATACGTTTAAAGAGTTGTTTCCAGCTTTTGAGAACAAAGAAGACAGGGCAACGGTTAATAATGCCATCCACAAT
TCAGCAGCAGTTTTATCTTCTACCCAGTTTGACGAGGTACTGAAACGCGACGAGCCACAGAAGAAAGAAGTTATTTTCGT
TACGGGAATTCCGGGTTCGGGAAAAACTTCGACAGTAAAAAACATGATGATGCAGGATACCACCAAGCTGCTTTTTGAAG
GACAGCTTGCACGGCCGCAGTCTGCTTTTCGCAAGATTGAGCAGTGTCTGGAGCGTAATCTTGAAGTTACTATCGTAGCC
GTCAGCATGAGGGCTGAAAGGGCTTCCGACAATACTTACAAACGGTTTAACGAATACGGCAGGGAAGCCAGTATAGGTAT
TATGGCTGATATTCAGGCCAATCTTCCTGACGGACTCAAGCAAATCAGGGATAAGTTTGGTGATGCGGTAAAAATTGTCG
GTATCAACCAAGACAGGAACAGTGAATTTATCGACAAGTTCGATGATGTCATCAAAATGCTTTCCTTGGGGTCGCAAGAG
CAAATATTGGGCAGGTTGGCCGAGAAAATCCAGAGCGACTTCGACTCAGGAAAAATCAGCCGGGAATGCTTCAATCAGGC
CAAGGGCAGTATGGATCTTGAAAGTGTTTTCGCCAAAAAAGAGTATTCCCAGCAAAGGGTAGTTACTAACTCCAAGGGTG
TTACCCTAGAAACAAAGTCAGCAAACGAGTTGTGGTCTAAGGTGGAACAAATTCCTGTAACAGGCATGAAAGCCGGTATC
TATTTACTAGGTCAAGCTAAAAAAGCAGAGACTGGTCAAACTTATTCCGGAGAAATCATTTACAAGGATGCCGCTGCGGT
ATTTCAAAAAACCAAAAACGGGCTTGTCAGGCACAATGCAACACATAACGAAGAAAGACTGGCCAAATTGGTTGAGATAG
GGCAGAACGTTTCTATCGGAAGCAATAAGGGCAAATTGATTGTCAAGTCTTTGGAGTATTCCGCCAAAAAATCCATCAGC
AGATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT33186 WP_003701903.1 NZ_AP019854:c12461-12030 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT33186 NZ_AP019854:c12461-12030 [Neisseria gonorrhoeae]
ATGAAAGAAAAAATGCGGTTGATCACGGAAATTTCGCTGATTATCAGGGAACTGTCTTATTCCCGGCGCGGGTATCGGCC
TGAGAGTTATGCGGCTGCGCTGGATAATTTGACGATAATCGGAGATTTGGCCGACATTTTGCACAACATCGAGGCAGCGA
CAGGCAATCCATTTGTTCAAAGGATGGTTGATGAAAAACTGAGAAAGTTTGTTACCGATTTTCCGGCTTATAAGGTACGG
TTGTCTCCGTTTTTGAATGGCTGTCCCCATGACCTTTCGTTAGGAGCAGAATCGTTGGGTAAGGGGGAGAGTATCGCTGA
TGCCTTGGCCGTTCCTCCGGAAGAGGTAGATATTGCAGACATTGAGTTTGACCGTGTGCTTATCGAAGACGACCGTTGGA
CAGGAGCATATGATGGTGGTAACGCGCAATGA

Structures

Antitoxin

• AlphaFold DB: D5K9G8