Detailed information of TA system TA033180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 1828334..1828881
• Replicon: chromosome
• Accession: NZ_AP019849
• Organism: Vibrio alfacsensis strain 04Ya249

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K5706_RS08125
• Protein ID: WP_015296906.1
• Coordinates: 1828334..1828636 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7Y3ZEQ4
• Locus tag: K5706_RS08130
• Protein ID: WP_015296907.1
• Coordinates: 1828624..1828881 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5706_RS08070	1824259..1824351	-	93	WP_078532186.1	DUF3265 domain-containing protein	-
K5706_RS08075 (VA249_15330)	1824426..1824671	+	246	WP_038140558.1	type II toxin-antitoxin system CcdA family antitoxin	-
K5706_RS08080 (VA249_15340)	1824671..1824988	+	318	WP_221069474.1	CcdB family protein	-
K5706_RS23805	1825050..1825133	-	84	Protein_1519	DUF3265 domain-containing protein	-
K5706_RS08085 (VA249_15350)	1825114..1825899	-	786	WP_050933286.1	hypothetical protein	-
K5706_RS08090	1825948..1826037	-	90	WP_079750657.1	DUF3265 domain-containing protein	-
K5706_RS08095 (VA249_15360)	1826067..1826543	-	477	WP_042765616.1	GNAT family N-acetyltransferase	-
K5706_RS08100 (VA249_15370)	1826696..1827016	-	321	WP_128811053.1	hypothetical protein	-
K5706_RS08105	1827130..1827222	-	93	WP_080264546.1	DUF3265 domain-containing protein	-
K5706_RS23810 (VA249_15380)	1827237..1827848	-	612	WP_225923898.1	hypothetical protein	-
K5706_RS08115 (VA249_15390)	1827943..1828176	-	234	WP_221069476.1	hypothetical protein	-
K5706_RS08120	1828206..1828298	-	93	WP_079858050.1	DUF3265 domain-containing protein	-
K5706_RS08125 (VA249_15400)	1828334..1828636	-	303	WP_015296906.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5706_RS08130 (VA249_15410)	1828624..1828881	-	258	WP_015296907.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K5706_RS08135 (VA249_15430)	1829632..1830393	+	762	WP_221069479.1	IS5 family transposase	-
K5706_RS08140	1830571..1830660	-	90	WP_072609953.1	DUF3265 domain-containing protein	-
K5706_RS23815	1830828..1831091	+	264	WP_225923924.1	hypothetical protein	-
K5706_RS08145	1831191..1831322	-	132	WP_130241604.1	DUF3265 domain-containing protein	-
K5706_RS08150 (VA249_15440)	1831846..1832214	-	369	WP_221069480.1	hypothetical protein	-
K5706_RS08155	1832241..1832351	-	111	WP_221071759.1	DUF3265 domain-containing protein	-
K5706_RS08160 (VA249_15450)	1832348..1832662	-	315	WP_221069481.1	DNA-binding transcriptional regulator	-
K5706_RS08165 (VA249_15460)	1832649..1832981	-	333	WP_012600340.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K5706_RS08170	1833084..1833173	-	90	WP_072615218.1	DUF3265 domain-containing protein	-
K5706_RS08175 (VA249_15470)	1833287..1833529	+	243	WP_005442033.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K5706_RS08180 (VA249_15480)	1833537..1833836	+	300	WP_221069483.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1823303..1823818	515
	-	flank	IS/Tn	-	-	1829632..1830393	761
	-	inside	Integron	-	-	1817009..1865094	48085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11727.61 Da
• Isoelectric Point: 4.8838

>T33180 WP_015296906.1 NZ_AP019849:c1828636-1828334 [Vibrio alfacsensis]
MAEIIWTEPALSDLNDIAEYIALENIVAAKQLVQTIFSKVERLQTFPESGRIPPELEHLSYREVVVNPCRVFYKQDDDKV
FILFVMRAERDLRKFLLSKQ

Nucleotide

• Length: 303 bp

>T33180 NZ_AP019849:c1828636-1828334 [Vibrio alfacsensis]
ATGGCTGAAATAATCTGGACTGAACCAGCGTTATCTGACCTCAACGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAGCAGTTAGTGCAAACGATCTTCTCCAAAGTCGAACGCCTACAAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGAGCTAGAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGATGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAGATTTGCGTAAGTTCCTGTTGAGTAAACAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9638.16 Da
• Isoelectric Point: 6.7269

>AT33180 WP_015296907.1 NZ_AP019849:c1828881-1828624 [Vibrio alfacsensis]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLVDVDDYEFMQNRLAILEGIARGERALADGKMVSHDEAKDKM
SKWLK

Nucleotide

• Length: 258 bp

>AT33180 NZ_AP019849:c1828881-1828624 [Vibrio alfacsensis]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATCCTCGCCGATCTCCACGATACCAAAGAGCCAGT
GTTAATTACTGAGCATGGTAAGCCATCCGCTTATCTCGTTGATGTTGATGACTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGACGGTAAAATGGTAAGCCATGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7Y3ZEQ4