Detailed information of TA system TA033176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF-MazE
• Location: 350870..351444
• Replicon: chromosome
• Accession: NZ_AP019846
• Organism: Leptotrichia hongkongensis strain JMUB5056

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FVE75_RS01720
• Protein ID: WP_147004908.1
• Coordinates: 351115..351444 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: U2WM84
• Locus tag: FVE75_RS01715
• Protein ID: WP_021769887.1
• Coordinates: 350870..351121 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE75_RS11040	345973..346314	+	342	WP_197735198.1	DDE-type integrase/transposase/recombinase	-
FVE75_RS01700	347224..348234	+	1011	WP_147004905.1	heat-inducible transcriptional repressor HrcA	-
FVE75_RS01705	348259..348846	+	588	WP_147004906.1	nucleotide exchange factor GrpE	-
FVE75_RS01710	348912..350735	+	1824	WP_147004907.1	molecular chaperone DnaK	-
FVE75_RS01715	350870..351121	+	252	WP_021769887.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FVE75_RS01720	351115..351444	+	330	WP_147004908.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FVE75_RS01725	351511..352392	+	882	WP_147004909.1	aldose 1-epimerase family protein	-
FVE75_RS01730	352575..353057	+	483	WP_147004910.1	phosphatidate cytidylyltransferase	-
FVE75_RS01735	353134..354306	+	1173	WP_147004911.1	molecular chaperone DnaJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-99)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12290.30 Da
• Isoelectric Point: 5.6261

>T33176 WP_147004908.1 NZ_AP019846:351115-351444 [Leptotrichia hongkongensis]
MVKQGDIIKINFNPQVGHEQAGYRPAVIVSNDTFNKVTNLVLVCPITNSIDKFPLHIKLDSRTATTGIILCEHLKSLDIT
TRSYKIIEKLPEDILEKVVDIIFSEVEIE

Nucleotide

• Length: 330 bp

>T33176 NZ_AP019846:351115-351444 [Leptotrichia hongkongensis]
ATGGTAAAACAAGGGGATATAATAAAGATAAATTTTAATCCGCAGGTAGGACATGAACAAGCTGGATATCGTCCTGCGGT
TATAGTAAGCAATGATACTTTCAATAAGGTAACAAATTTAGTTTTAGTTTGTCCGATTACAAATTCAATAGATAAATTTC
CGCTTCATATAAAATTAGACAGTCGGACTGCTACTACGGGTATAATCTTATGTGAACACCTTAAGTCTTTGGATATTACT
ACAAGAAGTTATAAAATTATTGAAAAATTACCAGAGGACATTTTAGAAAAAGTTGTAGACATTATTTTTTCTGAAGTAGA
AATTGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9815.45 Da
• Isoelectric Point: 6.3796

>AT33176 WP_021769887.1 NZ_AP019846:350870-351121 [Leptotrichia hongkongensis]
MAEVLKIQKWGNSQGIRLPKKILEILDLKVNDTILIEEGDDCLKLKKIKKEKRKTIKELFANYNEDYEKQEIDWGEPVGK
EVW

Nucleotide

• Length: 252 bp

>AT33176 NZ_AP019846:350870-351121 [Leptotrichia hongkongensis]
ATGGCAGAGGTTTTAAAAATACAAAAATGGGGAAACAGTCAAGGAATAAGACTTCCTAAAAAAATATTGGAAATACTTGA
TTTAAAGGTAAATGACACTATTTTGATAGAAGAGGGAGATGATTGCCTTAAATTAAAAAAAATAAAAAAAGAAAAAAGAA
AGACAATAAAGGAATTATTTGCAAATTATAATGAGGATTATGAAAAACAGGAAATAGATTGGGGAGAACCAGTAGGTAAA
GAGGTATGGTAA

Structures

Antitoxin

• AlphaFold DB: U2WM84