Detailed information of TA system TA033163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 9382..9854
• Replicon: plasmid pJMUB3870-1
• Accession: NZ_AP019832
• Organism: Leptotrichia trevisanii strain JMUB3870

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JMUB4039_RS12965
• Protein ID: WP_155283284.1
• Coordinates: 9582..9854 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JMUB4039_RS12960
• Protein ID: WP_155283283.1
• Coordinates: 9382..9597 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMUB4039_RS12935	5576..5899	-	324	WP_155283281.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JMUB4039_RS12940	5899..6126	-	228	WP_018498936.1	hypothetical protein	-
JMUB4039_RS12945	6505..7152	+	648	WP_018450730.1	recombinase family protein	-
JMUB4039_RS12950	7280..7783	+	504	WP_155283282.1	type II toxin-antitoxin system PemK/MazF family toxin	-
JMUB4039_RS12955	7968..8156	+	189	WP_149201969.1	hypothetical protein	-
JMUB4039_RS12960	9382..9597	+	216	WP_155283283.1	antitoxin	Antitoxin
JMUB4039_RS12965	9582..9854	+	273	WP_155283284.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JMUB4039_RS12970	9988..10914	-	927	WP_155283285.1	replication initiation protein	-
JMUB4039_RS12975	11547..11888	+	342	WP_026748886.1	hypothetical protein	-
JMUB4039_RS12980	12107..12286	-	180	Protein_13	ankyrin repeat domain-containing protein	-
JMUB4039_RS12985	12623..13705	-	1083	WP_051354435.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25939	25939

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-88)

Antitoxin

• Protein of unknown function DUF6290 (1-70)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10662.50 Da
• Isoelectric Point: 10.7635

>T33163 WP_155283284.1 NZ_AP019832:9582-9854 [Leptotrichia trevisanii]
MGRTVKYKLIPTPHFAKDFSKLDEFVKKRIKIYLENIAEDPRSKGKMLKANRKGQWRYRIGNYRVIVNIQDENLVVLALE
VGHRKNIYNS

Nucleotide

• Length: 273 bp

>T33163 NZ_AP019832:9582-9854 [Leptotrichia trevisanii]
ATGGGAAGAACTGTAAAATATAAACTAATCCCAACTCCACATTTCGCAAAAGACTTTAGCAAACTAGATGAATTTGTAAA
AAAAAGAATAAAAATTTATTTAGAAAATATTGCAGAAGATCCACGCAGTAAAGGAAAAATGTTGAAAGCAAATAGAAAAG
GACAATGGAGATACAGAATAGGAAATTACAGAGTTATTGTAAATATTCAAGATGAAAATTTAGTAGTATTAGCGTTAGAA
GTAGGGCATAGAAAAAATATTTATAATAGTTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8154.35 Da
• Isoelectric Point: 4.4501

>AT33163 WP_155283283.1 NZ_AP019832:9382-9597 [Leptotrichia trevisanii]
MASISLKVSDMEKKFLQSMAQFEGVTLSELIKSKVFDSLEDEYDAKIADLRLSEYESYLKNGGKVLKWEEL

Nucleotide

• Length: 216 bp

>AT33163 NZ_AP019832:9382-9597 [Leptotrichia trevisanii]
ATGGCTTCTATAAGTTTAAAGGTGTCTGATATGGAAAAAAAATTTTTGCAAAGTATGGCACAATTTGAAGGAGTTACATT
GTCAGAGTTAATAAAATCAAAAGTGTTTGACTCACTAGAAGATGAATACGATGCCAAAATAGCAGATTTAAGATTATCAG
AATATGAAAGCTATTTGAAAAATGGAGGAAAAGTTTTAAAATGGGAAGAACTGTAA