Detailed information of TA system TA033150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 15568..16040
• Replicon: plasmid pJCM16775-1
• Accession: NZ_AP019824
• Organism: Leptotrichia hofstadii strain JCM16775

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U2RV34
• Locus tag: FVE77_RS12150
• Protein ID: WP_021745737.1
• Coordinates: 15568..15840 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C9N208
• Locus tag: FVE77_RS12155
• Protein ID: WP_006806162.1
• Coordinates: 15825..16040 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE77_RS12140	13436..14113	+	678	WP_146967971.1	hypothetical protein	-
FVE77_RS12145	14514..15434	+	921	WP_036087326.1	replication initiation protein	-
FVE77_RS12150	15568..15840	-	273	WP_021745737.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FVE77_RS12155	15825..16040	-	216	WP_006806162.1	hypothetical protein	Antitoxin
FVE77_RS12725	17262..17450	-	189	WP_172966428.1	hypothetical protein	-
FVE77_RS12160	17635..18138	-	504	WP_026745118.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FVE77_RS12165	18247..18912	-	666	WP_026745119.1	recombinase family protein	-
FVE77_RS12520	19208..19378	+	171	WP_154669765.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34135	34135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-88)

Antitoxin

• Protein of unknown function DUF6290 (1-70)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10691.54 Da
• Isoelectric Point: 10.6516

>T33150 WP_021745737.1 NZ_AP019824:c15840-15568 [Leptotrichia hofstadii]
MGRTVKYKIIPTPHFVKDFSKLDEFVKKRIKIYLENIAEDPRSKGKMLKANRKGQWRYRIGDYRVIVNIQDENLVVLALE
VGHRKNIYNS

Nucleotide

• Length: 273 bp

>T33150 NZ_AP019824:c15840-15568 [Leptotrichia hofstadii]
ATGGGAAGAACTGTAAAATATAAAATAATCCCAACTCCACATTTCGTAAAAGACTTTAGCAAACTAGATGAATTTGTAAA
AAAAAGAATAAAAATTTATTTAGAAAATATTGCAGAAGATCCACGCAGTAAAGGAAAAATGTTGAAAGCAAATAGAAAAG
GACAATGGAGATACAGAATAGGAGATTACAGAGTTATTGTAAATATCCAAGATGAAAATTTAGTAGTATTAGCGTTAGAA
GTAGGACATAGAAAAAATATTTATAATAGTTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8155.29 Da
• Isoelectric Point: 4.2346

>AT33150 WP_006806162.1 NZ_AP019824:c16040-15825 [Leptotrichia hofstadii]
MASISLKVSDMEKKFLQSMAQFEGVTLSELIKSKVFDSLEDEYDAKIADLRLSEYESYLKNGGEVLKWEEL

Nucleotide

• Length: 216 bp

>AT33150 NZ_AP019824:c16040-15825 [Leptotrichia hofstadii]
ATGGCTTCTATAAGTTTAAAAGTATCTGATATGGAAAAAAAATTTTTGCAAAGTATGGCGCAATTTGAAGGAGTTACATT
GTCAGAGTTAATAAAATCAAAAGTGTTTGACTCACTAGAAGATGAATACGATGCCAAAATAGCAGATTTAAGATTATCAG
AATATGAAAGCTATTTGAAAAATGGAGGAGAAGTTTTAAAATGGGAAGAACTGTAA

Structures

Toxin

• AlphaFold DB: A0A510KH77

Antitoxin

• AlphaFold DB: A0A510KIG5