Detailed information of TA system TA033129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2228996..2229735
• Replicon: chromosome
• Accession: NZ_AP019817
• Organism: Enterobacter hormaechei subsp. hoffmannii strain OIPH-N069

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A3S0FZ61
• Locus tag: G7Y81_RS10600
• Protein ID: WP_022648141.1
• Coordinates: 2228996..2229481 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: G7Y81_RS10605
• Protein ID: WP_003857131.1
• Coordinates: 2229469..2229735 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Y81_RS10575	2225152..2226174	+	1023	WP_022648135.1	alpha/beta hydrolase	-
G7Y81_RS10580	2226270..2227034	+	765	WP_022648136.1	SDR family oxidoreductase	-
G7Y81_RS10585	2227683..2227964	+	282	Protein_2058	DUF4225 domain-containing protein	-
G7Y81_RS10590	2227939..2228268	-	330	WP_022648139.1	hypothetical protein	-
G7Y81_RS10595	2228401..2228970	+	570	WP_022648140.1	hypothetical protein	-
G7Y81_RS10600	2228996..2229481	-	486	WP_022648141.1	GNAT family N-acetyltransferase	Toxin
G7Y81_RS10605	2229469..2229735	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
G7Y81_RS10610	2229799..2230728	-	930	WP_022648142.1	LysR family transcriptional regulator	-
G7Y81_RS10615	2230858..2232246	+	1389	WP_022648143.1	MFS transporter	-
G7Y81_RS10620	2232225..2232782	-	558	WP_022648144.1	OmpH family outer membrane protein	-
G7Y81_RS10625	2232903..2234039	-	1137	WP_022648145.1	type 1 fimbrial protein	-
G7Y81_RS10630	2234063..2234587	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2206129..2232782	26653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17558.24 Da
• Isoelectric Point: 9.6275

>T33129 WP_022648141.1 NZ_AP019817:c2229481-2228996 [Enterobacter hormaechei subsp. hoffmannii]
VGRVTPPEPLSSVHQLAEFISGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T33129 NZ_AP019817:c2229481-2228996 [Enterobacter hormaechei subsp. hoffmannii]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT33129 WP_003857131.1 NZ_AP019817:c2229735-2229469 [Enterobacter hormaechei subsp. hoffmannii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT33129 NZ_AP019817:c2229735-2229469 [Enterobacter hormaechei subsp. hoffmannii]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A3S0FZ61

Antitoxin

• AlphaFold DB: A0A837FCR9