Detailed information of TA system TA033123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1211798..1212288
• Replicon: chromosome
• Accession: NZ_AP019810
• Organism: Enterococcus mundtii strain 15-1A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V5XU14
• Locus tag: HZ311_RS05775
• Protein ID: WP_010734226.1
• Coordinates: 1211798..1212070 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V5XRI7
• Locus tag: HZ311_RS05780
• Protein ID: WP_010734225.1
• Coordinates: 1212067..1212288 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZ311_RS05760	1207209..1208975	-	1767	WP_010734229.1	aspartate--tRNA ligase	-
HZ311_RS05765	1208975..1210282	-	1308	WP_023520426.1	histidine--tRNA ligase	-
HZ311_RS05770	1210690..1211640	+	951	WP_137072868.1	aldo/keto reductase	-
HZ311_RS05775	1211798..1212070	-	273	WP_010734226.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZ311_RS05780	1212067..1212288	-	222	WP_010734225.1	hypothetical protein	Antitoxin
HZ311_RS05785	1212381..1213640	-	1260	WP_010734224.1	cytochrome P450	-
HZ311_RS05790	1214220..1214666	-	447	WP_178946527.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HZ311_RS05795	1214678..1216891	-	2214	WP_178946528.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-89)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10642.34 Da
• Isoelectric Point: 10.3153

>T33123 WP_010734226.1 NZ_AP019810:c1212070-1211798 [Enterococcus mundtii]
MRKQYLVRYEKGAQKALKKMDKFQASLILSWIEKNLINTDNPRQYGKGLVANRSGEWRYRVGNYRIIADIDDEVITILIV
QIGHRNDIYK

Nucleotide

• Length: 273 bp

>T33123 NZ_AP019810:c1212070-1211798 [Enterococcus mundtii]
ATGAGGAAGCAATATCTTGTCCGTTATGAAAAAGGTGCACAAAAAGCATTGAAAAAAATGGATAAATTTCAAGCTTCTTT
GATACTATCATGGATAGAAAAAAATCTTATCAATACAGATAACCCCAGACAATATGGTAAAGGGTTAGTAGCGAATAGGA
GTGGCGAATGGCGTTATAGAGTGGGGAATTATCGGATCATTGCAGATATAGATGATGAAGTGATAACGATTTTGATCGTA
CAAATTGGTCATCGAAATGACATCTATAAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8382.54 Da
• Isoelectric Point: 4.2571

>AT33123 WP_010734225.1 NZ_AP019810:c1212288-1212067 [Enterococcus mundtii]
MSNITFRVSDEEKAFMLAMADLNGMTVSELARTTLLETLEDQIDMDIYNKAMKDHKSLDESISHEEMKRELGL

Nucleotide

• Length: 222 bp

>AT33123 NZ_AP019810:c1212288-1212067 [Enterococcus mundtii]
ATGAGTAATATTACGTTTCGTGTATCAGACGAAGAAAAAGCATTTATGTTAGCAATGGCTGATTTGAATGGAATGACCGT
TTCAGAATTGGCAAGGACAACTTTATTAGAAACGCTAGAAGATCAGATTGATATGGATATCTATAATAAAGCGATGAAAG
ATCATAAGAGTCTTGATGAAAGTATCTCGCATGAAGAAATGAAAAGAGAACTAGGATTATGA

Structures

Toxin

• AlphaFold DB: V5XU14

Antitoxin

• AlphaFold DB: V5XRI7