Detailed information of TA system TA033122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1203595..1204204
• Replicon: chromosome
• Accession: NZ_AP019810
• Organism: Enterococcus mundtii strain 15-1A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HZ311_RS05745
• Protein ID: WP_010734231.1
• Coordinates: 1204016..1204204 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V5XTD1
• Locus tag: HZ311_RS05740
• Protein ID: WP_010734232.1
• Coordinates: 1203595..1203981 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZ311_RS05720	1199369..1200004	-	636	WP_010734236.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
HZ311_RS05725	1200136..1200870	+	735	WP_010734235.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
HZ311_RS05730	1200860..1201147	+	288	WP_010734234.1	GIY-YIG nuclease family protein	-
HZ311_RS05735	1201269..1203200	-	1932	WP_010734233.1	acetyltransferase	-
HZ311_RS05740	1203595..1203981	-	387	WP_010734232.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HZ311_RS05745	1204016..1204204	-	189	WP_010734231.1	type II toxin-antitoxin system HicA family toxin	Toxin
HZ311_RS05750	1205730..1205876	-	147	WP_155237725.1	hypothetical protein	-
HZ311_RS05755	1206126..1207001	-	876	WP_010734230.1	YitT family protein	-
HZ311_RS05760	1207209..1208975	-	1767	WP_010734229.1	aspartate--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-62)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-101)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6983.18 Da
• Isoelectric Point: 11.0565

>T33122 WP_010734231.1 NZ_AP019810:c1204204-1204016 [Enterococcus mundtii]
MPMTIREAEKLLKDAGFVEVKGGKGSHRKFTKKDHPRPVILTSHSKELSRRVEDSVRKAVGI

Nucleotide

• Length: 189 bp

>T33122 NZ_AP019810:c1204204-1204016 [Enterococcus mundtii]
ATGCCCATGACAATCAGAGAAGCAGAAAAGCTACTGAAAGATGCTGGCTTTGTTGAAGTGAAAGGTGGGAAGGGCTCACA
TAGGAAATTCACTAAAAAGGATCATCCTAGACCTGTTATCCTTACTAGCCATTCAAAAGAGTTATCTAGAAGAGTAGAAG
ATTCTGTTCGAAAAGCAGTGGGCATTTAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.41 Da
• Isoelectric Point: 4.4851

>AT33122 WP_010734232.1 NZ_AP019810:c1203981-1203595 [Enterococcus mundtii]
MKKVYPAIFEKDPVGYGIYFPDIEGAVTQSKDIIEGLEVASDALGIMLGDLVENNKPLPKPTNINDLSIDSEREFATLIS
VDLNDYLKDVQLDKKTIKIPHWLNVRATNEGINFSKTMSEALVQKLNI

Nucleotide

• Length: 387 bp

>AT33122 NZ_AP019810:c1203981-1203595 [Enterococcus mundtii]
ATGAAAAAAGTTTATCCAGCTATTTTTGAAAAGGATCCTGTTGGATATGGAATTTATTTTCCTGATATCGAAGGAGCAGT
GACACAATCAAAAGATATTATCGAGGGGTTAGAAGTTGCATCGGATGCTTTAGGGATCATGCTCGGTGATTTAGTTGAGA
ATAATAAACCTTTGCCAAAGCCAACTAATATCAATGATCTTTCGATTGATTCTGAGCGTGAATTTGCTACTTTGATTTCT
GTGGATCTAAATGATTACTTAAAAGATGTCCAACTAGATAAAAAGACAATAAAAATTCCTCATTGGTTAAATGTGCGCGC
AACAAATGAAGGGATAAATTTTTCAAAAACAATGTCTGAAGCTTTGGTTCAAAAATTAAATATATGA

Structures

Antitoxin

• AlphaFold DB: V5XTD1