Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 5149..5750 Replicon plasmid pKY1497_2
Accession NZ_AP019805
Organism Escherichia coli strain KY 1497

Toxin (Protein)


Gene name doc Uniprot ID A0A6G4LZ59
Locus tag ECKY1497_RS25500 Protein ID WP_001216040.1
Coordinates 5149..5529 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID I2USI4
Locus tag ECKY1497_RS25505 Protein ID WP_001190710.1
Coordinates 5529..5750 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECKY1497_RS25465 (ECKY1497_P20010) 168..1196 + 1029 WP_032323253.1 tyrosine-type recombinase/integrase -
ECKY1497_RS25470 (ECKY1497_P20020) 1270..1614 + 345 WP_248520507.1 hypothetical protein -
ECKY1497_RS25475 (ECKY1497_P20030) 1611..2087 + 477 WP_032323256.1 hypothetical protein -
ECKY1497_RS25480 (ECKY1497_P20040) 2084..2578 + 495 WP_032323258.1 dUTP diphosphatase -
ECKY1497_RS25485 (ECKY1497_P20050) 2593..3255 + 663 WP_032152851.1 DUF2829 domain-containing protein -
ECKY1497_RS25490 (ECKY1497_P20060) 3262..4029 + 768 WP_032323262.1 hypothetical protein -
ECKY1497_RS25495 (ECKY1497_P20070) 4019..5113 + 1095 WP_181464646.1 hypothetical protein -
ECKY1497_RS25500 (ECKY1497_P20080) 5149..5529 - 381 WP_001216040.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ECKY1497_RS25505 (ECKY1497_P20090) 5529..5750 - 222 WP_001190710.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
ECKY1497_RS25510 (ECKY1497_P20100) 5823..6212 - 390 WP_000506724.1 S24 family peptidase -
ECKY1497_RS25515 (ECKY1497_P20110) 6387..6971 + 585 WP_044068914.1 hypothetical protein -
ECKY1497_RS25520 (ECKY1497_P20120) 6972..7334 + 363 WP_001062543.1 hypothetical protein -
ECKY1497_RS25525 (ECKY1497_P20130) 7346..7585 - 240 WP_023356286.1 DNA polymerase III subunit theta -
ECKY1497_RS25530 (ECKY1497_P20140) 7588..7854 - 267 WP_000903205.1 hypothetical protein -
ECKY1497_RS25535 (ECKY1497_P20150) 7900..8610 - 711 WP_181464647.1 hypothetical protein -
ECKY1497_RS25540 8607..8816 - 210 WP_032323266.1 hypothetical protein -
ECKY1497_RS25545 (ECKY1497_P20160) 8841..9134 - 294 WP_032323267.1 hypothetical protein -
ECKY1497_RS25550 (ECKY1497_P20170) 9158..9439 - 282 WP_248520509.1 ASCH domain-containing protein -
ECKY1497_RS25555 9432..9674 - 243 Protein_18 DUF551 domain-containing protein -
ECKY1497_RS25560 10005..10223 - 219 WP_248520511.1 DUF4014 family protein -
ECKY1497_RS25565 (ECKY1497_P20190) 10225..10716 - 492 WP_248520514.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..93491 93491


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13678.59 Da        Isoelectric Point: 6.2282

>T33115 WP_001216040.1 NZ_AP019805:c5529-5149 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDDPVLVELAVGAATGEIPVSSVAKKLRELYGSNI

Download         Length: 381 bp

>T33115 NZ_AP019805:c5529-5149 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTGTTTCTACGCCGTAACGGTATACAGGTATATGATGATCCCGTGCTGGTGGAGCTTGCAGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGAAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT33115 WP_001190710.1 NZ_AP019805:c5750-5529 [Escherichia coli]
MQSINFRTARGNLSELLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT33115 NZ_AP019805:c5750-5529 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAATTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAACCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAGGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4LZ59

Antitoxin

Source ID Structure
AlphaFold DB A0A348ERB2

References