Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/ElaA-DUF1778 |
| Location | 31393..32196 | Replicon | plasmid pKY1497_1 |
| Accession | NZ_AP019804 | ||
| Organism | Escherichia coli strain KY 1497 | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | G3CAI8 |
| Locus tag | ECKY1497_RS24940 | Protein ID | WP_000348883.1 |
| Coordinates | 31666..32196 (+) | Length | 177 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | G3CAI7 |
| Locus tag | ECKY1497_RS24935 | Protein ID | WP_001275013.1 |
| Coordinates | 31393..31662 (+) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECKY1497_RS24905 (26502) | 26502..27242 | + | 741 | WP_029702141.1 | tyrosine-type recombinase/integrase | - |
| ECKY1497_RS24910 (27538) | 27538..27806 | + | 269 | Protein_35 | type II toxin-antitoxin system ParD family antitoxin | - |
| ECKY1497_RS24915 (27793) | 27793..28083 | + | 291 | Protein_36 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| ECKY1497_RS24920 (28150) | 28150..28332 | + | 183 | WP_042004909.1 | hypothetical protein | - |
| ECKY1497_RS24925 (28453) | 28453..29193 | + | 741 | WP_001586233.1 | tyrosine-type recombinase/integrase | - |
| ECKY1497_RS24930 (29516) | 29516..30505 | - | 990 | WP_123002471.1 | RepB family plasmid replication initiator protein | - |
| ECKY1497_RS24935 (31393) | 31393..31662 | + | 270 | WP_001275013.1 | DUF1778 domain-containing protein | Antitoxin |
| ECKY1497_RS24940 (31666) | 31666..32196 | + | 531 | WP_000348883.1 | GNAT family N-acetyltransferase | Toxin |
| ECKY1497_RS24945 (32197) | 32197..32414 | + | 218 | Protein_42 | transposase | - |
| ECKY1497_RS24950 (32470) | 32470..32786 | - | 317 | Protein_43 | type II toxin-antitoxin system VapC family toxin | - |
| ECKY1497_RS24955 (32783) | 32783..33013 | - | 231 | WP_001261274.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| ECKY1497_RS24960 (32970) | 32970..33068 | + | 99 | Protein_45 | hypothetical protein | - |
| ECKY1497_RS24965 (33212) | 33212..35833 | - | 2622 | WP_001586230.1 | hypothetical protein | - |
| ECKY1497_RS24970 (36224) | 36224..36725 | + | 502 | Protein_47 | HEPN family nuclease | - |
| ECKY1497_RS24975 (36738) | 36738..37175 | + | 438 | WP_001403959.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) / blaTEM-1B / tet(B) | - | 1..123767 | 123767 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 177 a.a. Molecular weight: 20321.20 Da Isoelectric Point: 6.4889
>T33111 WP_000348883.1 NZ_AP019804:31666-32196 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE
Download Length: 531 bp
>T33111 NZ_AP019804:31666-32196 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10229.63 Da Isoelectric Point: 6.4702
>AT33111 WP_001275013.1 NZ_AP019804:31393-31662 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE
Download Length: 270 bp
>AT33111 NZ_AP019804:31393-31662 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|