Detailed information of TA system TA033110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 5052759..5053573
• Replicon: chromosome
• Accession: NZ_AP019803
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: ECKY1497_RS24195
• Protein ID: WP_001054376.1
• Coordinates: 5053316..5053573 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: ECKY1497_RS24190
• Protein ID: WP_001309181.1
• Coordinates: 5052759..5053304 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS24160 (5048450)	5048450..5049763	-	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-
ECKY1497_RS24165 (5049775)	5049775..5050053	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
ECKY1497_RS24170 (5050050)	5050050..5051171	-	1122	WP_000010829.1	M42 family metallopeptidase	-
ECKY1497_RS24175 (5051416)	5051416..5051532	-	117	Protein_4725	VOC family protein	-
ECKY1497_RS24180 (5051570)	5051570..5051788	-	219	Protein_4726	hypothetical protein	-
ECKY1497_RS24185 (5051957)	5051957..5052703	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
ECKY1497_RS24190 (5052759)	5052759..5053304	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
ECKY1497_RS24195 (5053316)	5053316..5053573	-	258	WP_001054376.1	YjhX family toxin	Toxin
ECKY1497_RS24200 (5054064)	5054064..5054195	-	132	WP_001309182.1	hypothetical protein	-
ECKY1497_RS24205 (5054311)	5054311..5055551	+	1241	Protein_4731	helicase YjhR	-
ECKY1497_RS24210 (5055819)	5055819..5056024	-	206	Protein_4732	HNH endonuclease	-
ECKY1497_RS24215 (5056134)	5056134..5057114	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
ECKY1497_RS24220 (5057179)	5057179..5058285	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T33110 WP_001054376.1 NZ_AP019803:c5053573-5053316 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T33110 NZ_AP019803:c5053573-5053316 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT33110 WP_001309181.1 NZ_AP019803:c5053304-5052759 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT33110 NZ_AP019803:c5053304-5052759 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8