Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4937289..4937884 Replicon chromosome
Accession NZ_AP019803
Organism Escherichia coli strain KY 1497

Toxin (Protein)


Gene name chpB Uniprot ID V0SXT4
Locus tag ECKY1497_RS23605 Protein ID WP_000239581.1
Coordinates 4937534..4937884 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag ECKY1497_RS23600 Protein ID WP_001223213.1
Coordinates 4937289..4937540 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECKY1497_RS23590 (4932954) 4932954..4936733 + 3780 WP_001522396.1 autotransporter assembly complex protein TamB -
ECKY1497_RS23595 (4936736) 4936736..4937077 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
ECKY1497_RS23600 (4937289) 4937289..4937540 + 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
ECKY1497_RS23605 (4937534) 4937534..4937884 + 351 WP_000239581.1 endoribonuclease toxin ChpB Toxin
ECKY1497_RS23610 (4937963) 4937963..4938493 - 531 WP_000055075.1 inorganic diphosphatase -
ECKY1497_RS23615 (4938803) 4938803..4939759 + 957 WP_001522398.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
ECKY1497_RS23620 (4939892) 4939892..4941394 + 1503 WP_001522399.1 sugar ABC transporter ATP-binding protein -
ECKY1497_RS23625 (4941408) 4941408..4942430 + 1023 WP_001361374.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12453.41 Da        Isoelectric Point: 6.2206

>T33108 WP_000239581.1 NZ_AP019803:4937534-4937884 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T33108 NZ_AP019803:4937534-4937884 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT33108 WP_001223213.1 NZ_AP019803:4937289-4937540 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT33108 NZ_AP019803:4937289-4937540 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SXT4

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References