Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4774361..4774858 Replicon chromosome
Accession NZ_AP019803
Organism Escherichia coli strain KY 1497

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECKY1497_RS22750 Protein ID WP_001173343.1
Coordinates 4774685..4774858 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECKY1497_RS22745 Protein ID WP_000398619.1
Coordinates 4774361..4774657 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECKY1497_RS22725 (4771090) 4771090..4771809 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECKY1497_RS22730 (4771806) 4771806..4773437 - 1632 WP_001216477.1 sensor histidine kinase -
ECKY1497_RS22735 (4773618) 4773618..4773848 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
ECKY1497_RS22740 (4773860) 4773860..4774132 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
ECKY1497_RS22745 (4774361) 4774361..4774657 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECKY1497_RS22750 (4774685) 4774685..4774858 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECKY1497_RS22755 (4774977) 4774977..4776494 - 1518 WP_000003806.1 lysine--tRNA ligase -
ECKY1497_RS22760 (4776731) 4776731..4778188 - 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T33106 WP_001173343.1 NZ_AP019803:4774685-4774858 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T33106 NZ_AP019803:4774685-4774858 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT33106 WP_000398619.1 NZ_AP019803:4774361-4774657 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT33106 NZ_AP019803:4774361-4774657 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References