Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3305656..3306310 Replicon chromosome
Accession NZ_AP019803
Organism Escherichia coli strain KY 1497

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ECKY1497_RS15820 Protein ID WP_000244781.1
Coordinates 3305656..3306063 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECKY1497_RS15825 Protein ID WP_000354046.1
Coordinates 3306044..3306310 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECKY1497_RS15800 (3301613) 3301613..3303346 - 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -
ECKY1497_RS15805 (3303352) 3303352..3304062 - 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECKY1497_RS15810 (3304087) 3304087..3304983 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECKY1497_RS15815 (3305095) 3305095..3305616 + 522 WP_001521233.1 flavodoxin FldB -
ECKY1497_RS15820 (3305656) 3305656..3306063 - 408 WP_000244781.1 protein YgfX Toxin
ECKY1497_RS15825 (3306044) 3306044..3306310 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECKY1497_RS15830 (3306553) 3306553..3307533 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
ECKY1497_RS15835 (3307610) 3307610..3308269 - 660 WP_000250269.1 hemolysin III family protein -
ECKY1497_RS15840 (3308433) 3308433..3308744 - 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
ECKY1497_RS15845 (3308789) 3308789..3310222 + 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T33101 WP_000244781.1 NZ_AP019803:c3306063-3305656 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T33101 NZ_AP019803:c3306063-3305656 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT33101 WP_000354046.1 NZ_AP019803:c3306310-3306044 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT33101 NZ_AP019803:c3306310-3306044 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References