Detailed information of TA system TA033097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1787290..1788251
• Replicon: chromosome
• Accession: NZ_AP019803
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1Q5ZNB7
• Locus tag: ECKY1497_RS08440
• Protein ID: WP_021523084.1
• Coordinates: 1787290..1787985 (-)
• Length: 232 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: ECKY1497_RS08445
• Protein ID: WP_001296726.1
• Coordinates: 1787985..1788251 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS08420 (1782778)	1782778..1783086	-	309	WP_001221046.1	hypothetical protein	-
ECKY1497_RS08425 (1783225)	1783225..1784124	+	900	WP_001523294.1	LysR family transcriptional regulator	-
ECKY1497_RS08430 (1784491)	1784491..1785523	+	1033	Protein_1648	ISNCY family transposase	-
ECKY1497_RS08435 (1785738)	1785738..1787090	+	1353	WP_001551127.1	SIR2 family protein	-
ECKY1497_RS08440 (1787290)	1787290..1787985	-	696	WP_021523084.1	HipA N-terminal domain-containing protein	Toxin
ECKY1497_RS08445 (1787985)	1787985..1788251	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1784705..1785523	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 232 a.a.
• Molecular weight: 25773.79 Da
• Isoelectric Point: 9.9928

>T33097 WP_021523084.1 NZ_AP019803:c1787985-1787290 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETIQAGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKI
YPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 696 bp

>T33097 NZ_AP019803:c1787985-1787290 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
TGAAACTATTCAGGCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCG
CAGGAATACACATCAGCGATCTCAAACTGGCAATGGGGGTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATT
TATCCGCGACATTTTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGC
CAGAATGATTCCGGCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTG
AAACCAATGTGTTGAGGTTGCACGGCCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT33097 WP_001296726.1 NZ_AP019803:c1788251-1787985 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT33097 NZ_AP019803:c1788251-1787985 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A1Q5ZNB7