Detailed information of TA system TA033096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1684819..1685457
• Replicon: chromosome
• Accession: NZ_AP019803
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: ECKY1497_RS07995
• Protein ID: WP_001306887.1
• Coordinates: 1684819..1684995 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECKY1497_RS08000
• Protein ID: WP_001270286.1
• Coordinates: 1685041..1685457 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS07970 (1679875)	1679875..1680333	+	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-
ECKY1497_RS07975 (1680441)	1680441..1681652	-	1212	WP_023143671.1	BenE family transporter YdcO	-
ECKY1497_RS07980 (1681705)	1681705..1682241	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
ECKY1497_RS07985 (1682314)	1682314..1684275	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECKY1497_RS07990 (1684367)	1684367..1684597	-	231	WP_000494241.1	YncJ family protein	-
ECKY1497_RS07995 (1684819)	1684819..1684995	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECKY1497_RS08000 (1685041)	1685041..1685457	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECKY1497_RS08005 (1685536)	1685536..1686942	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
ECKY1497_RS08010 (1687187)	1687187..1688332	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
ECKY1497_RS08015 (1688350)	1688350..1689363	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
ECKY1497_RS08020 (1689364)	1689364..1690305	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1679875..1680333	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T33096 WP_001306887.1 NZ_AP019803:1684819-1684995 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T33096 NZ_AP019803:1684819-1684995 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT33096 WP_001270286.1 NZ_AP019803:1685041-1685457 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT33096 NZ_AP019803:1685041-1685457 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76