Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 497991..498609 Replicon chromosome
Accession NZ_AP019803
Organism Escherichia coli strain KY 1497

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECKY1497_RS02310 Protein ID WP_001291435.1
Coordinates 497991..498209 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECKY1497_RS02315 Protein ID WP_000344800.1
Coordinates 498235..498609 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECKY1497_RS02275 (493280) 493280..493852 + 573 WP_000779831.1 YbaY family lipoprotein -
ECKY1497_RS02280 (493883) 493883..494194 - 312 WP_000409911.1 MGMT family protein -
ECKY1497_RS02290 (494573) 494573..494926 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECKY1497_RS02295 (494968) 494968..496518 - 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECKY1497_RS02300 (496682) 496682..497152 - 471 WP_001490888.1 YlaC family protein -
ECKY1497_RS02305 (497268) 497268..497819 - 552 WP_001522034.1 maltose O-acetyltransferase -
ECKY1497_RS02310 (497991) 497991..498209 - 219 WP_001291435.1 HHA domain-containing protein Toxin
ECKY1497_RS02315 (498235) 498235..498609 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECKY1497_RS02320 (499155) 499155..502304 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
ECKY1497_RS02325 (502327) 502327..503520 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33084 WP_001291435.1 NZ_AP019803:c498209-497991 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T33084 NZ_AP019803:c498209-497991 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT33084 WP_000344800.1 NZ_AP019803:c498609-498235 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT33084 NZ_AP019803:c498609-498235 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References