Detailed information of TA system TA033078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1168384..1169468
• Replicon: chromosome
• Accession: NZ_AP019801
• Organism: Thermus thermophilus strain HC11

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: TthHC11_RS06285
• Protein ID: WP_011173231.1
• Coordinates: 1168857..1169468 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: Q72JH4
• Locus tag: TthHC11_RS06280
• Protein ID: WP_011173230.1
• Coordinates: 1168384..1168797 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TthHC11_RS06255	1163434..1164378	+	945	WP_143591215.1	TAXI family TRAP transporter solute-binding subunit	-
TthHC11_RS06260	1164441..1166414	+	1974	WP_143591217.1	TRAP transporter permease	-
TthHC11_RS06265	1166430..1167155	+	726	WP_172967309.1	amino acid ABC transporter ATP-binding protein	-
TthHC11_RS06270	1167162..1167578	+	417	WP_011228478.1	hypothetical protein	-
TthHC11_RS06275	1167553..1168380	-	828	WP_143591219.1	patatin-like phospholipase family protein	-
TthHC11_RS06280	1168384..1168797	-	414	WP_011173230.1	helix-turn-helix domain-containing protein	Antitoxin
TthHC11_RS06285	1168857..1169468	-	612	WP_011173231.1	PIN domain-containing protein	Toxin
TthHC11_RS06290	1169495..1170259	+	765	WP_143591221.1	indole-3-glycerol phosphate synthase TrpC	-
TthHC11_RS06295	1170264..1170971	+	708	WP_143591223.1	1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase	-
TthHC11_RS06300	1170959..1171276	+	318	WP_011228484.1	DUF1049 domain-containing protein	-
TthHC11_RS06305	1171273..1173273	+	2001	WP_143591225.1	single-stranded-DNA-specific exonuclease RecJ	-
TthHC11_RS06310	1173245..1173808	+	564	WP_011173236.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-121)

Antitoxin

• Helix-turn-helix domain, group 17 (67-114)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 23534.41 Da
• Isoelectric Point: 6.1323

>T33078 WP_011173231.1 NZ_AP019801:c1169468-1168857 [Thermus thermophilus]
MARPLPQTLFLDACVLYPAPIRDLFMGLALEGLVRLKWNRRVQEEWIHNLLQDRPDLTPEQQARIRATPEKMKEVLAFQE
PLVEGYESLVEKIQLPDANDRHVVAAAWWGKAEAILTFNLKDFPEEELGRWELLALHPDDYLTELAERLIRKNFLPDPLL
RVLKRQRCALRKPPLGVEDFLEILRRAGLATFTEALGPYKGHL

Nucleotide

• Length: 612 bp

>T33078 NZ_AP019801:c1169468-1168857 [Thermus thermophilus]
ATGGCCCGCCCTCTTCCCCAAACCCTCTTTTTGGACGCCTGCGTCCTCTACCCCGCTCCCATCCGGGACCTTTTCATGGG
CCTGGCCTTGGAGGGACTTGTGCGGCTCAAGTGGAACCGGCGCGTCCAGGAGGAGTGGATCCACAACCTGCTTCAGGACC
GCCCTGACCTCACCCCGGAGCAGCAGGCGCGGATCAGGGCCACCCCTGAAAAGATGAAGGAGGTTTTGGCGTTCCAAGAA
CCCCTAGTAGAGGGCTACGAAAGCCTGGTGGAGAAAATCCAGCTCCCCGATGCAAACGACCGCCATGTGGTGGCCGCCGC
CTGGTGGGGAAAAGCAGAGGCCATCCTTACCTTCAACCTCAAGGATTTCCCCGAAGAGGAGCTTGGCCGCTGGGAACTCC
TGGCCCTCCACCCCGACGACTACCTTACGGAGCTCGCCGAAAGGCTGATCCGGAAAAACTTCCTGCCCGACCCCCTTCTC
AGGGTCCTTAAGCGCCAGCGGTGCGCCTTGCGCAAACCCCCGCTCGGGGTGGAGGACTTCTTGGAAATCCTGCGACGGGC
CGGGTTGGCCACCTTTACCGAGGCGCTTGGCCCCTACAAGGGGCACCTCTAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15107.62 Da
• Isoelectric Point: 8.5332

>AT33078 WP_011173230.1 NZ_AP019801:c1168797-1168384 [Thermus thermophilus]
MGGLLFTSTSSVEAVKAFREKLAPGKVLRLEEASLVLTPELARLFQELLGALLRGEAVRIVPLEAELTTGQAAELLGVSR
PYLVRLLEEGKIPHHKVGTHRRVRARDLLAYLEASRKRGRELLDELIGEAQELGLGY

Nucleotide

• Length: 414 bp

>AT33078 NZ_AP019801:c1168797-1168384 [Thermus thermophilus]
ATGGGCGGTCTCCTCTTCACCTCTACGTCCTCGGTGGAGGCGGTTAAGGCCTTCCGGGAAAAGCTTGCCCCCGGGAAGGT
TCTGCGCCTCGAGGAAGCCTCTTTGGTGCTCACCCCGGAGCTGGCCCGGCTTTTCCAGGAGCTTTTGGGGGCGCTTTTGC
GGGGTGAGGCGGTGCGCATCGTTCCCTTGGAAGCCGAGCTCACCACGGGCCAGGCGGCCGAGCTCCTCGGGGTCTCCCGG
CCTTACCTGGTCCGGCTTTTGGAGGAAGGCAAGATTCCCCACCACAAGGTGGGGACCCACCGCCGGGTCCGGGCCAGGGA
CCTTCTGGCCTACCTGGAGGCCTCCCGGAAGCGGGGCAGGGAGCTTTTGGACGAGCTCATCGGCGAGGCCCAAGAGCTCG
GCCTGGGGTATTAG

Structures

Antitoxin

• AlphaFold DB: Q72JH4