Detailed information of TA system TA033066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 380276..380888
• Replicon: chromosome
• Accession: NZ_AP019797
• Organism: Rhodothermus marinus strain AA3-38

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GYH26_RS01755
• Protein ID: WP_161540238.1
• Coordinates: 380499..380888 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GYH26_RS01750
• Protein ID: WP_161540237.1
• Coordinates: 380276..380506 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH26_RS01725	375387..376445	-	1059	WP_161542344.1	2-oxoacid:ferredoxin oxidoreductase subunit beta	-
GYH26_RS01730	376620..378482	-	1863	WP_161540234.1	2-oxoacid:acceptor oxidoreductase subunit alpha	-
GYH26_RS01735	378639..379022	+	384	WP_161540235.1	Co2+/Mg2+ efflux protein ApaG	-
GYH26_RS01740	379026..379286	-	261	WP_054684982.1	hypothetical protein	-
GYH26_RS01745	379368..380219	+	852	WP_161540236.1	thymidylate synthase	-
GYH26_RS01750	380276..380506	+	231	WP_161540237.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GYH26_RS01755	380499..380888	+	390	WP_161540238.1	type II toxin-antitoxin system VapC family toxin	Toxin
GYH26_RS01760	380899..381441	+	543	WP_161540239.1	dihydrofolate reductase	-
GYH26_RS01765	381551..384517	+	2967	WP_161540240.1	ribonucleoside reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (6-129)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-47)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14900.38 Da
• Isoelectric Point: 5.2472

>T33066 WP_161540238.1 NZ_AP019797:380499-380888 [Rhodothermus marinus]
MPERYLLDTHVLLWVLFSPERIPERVRERLIDPYFEVYFSAASVWEIAIKAQIGRLKLTLDLNAIVEAAEDSGFEPLAIR
TEHATKIIDLPLYHRDPFDRILVAQAIVEKARLLTADSALIKYKNVELI

Nucleotide

• Length: 390 bp

>T33066 NZ_AP019797:380499-380888 [Rhodothermus marinus]
ATGCCTGAGCGCTATTTGCTCGATACACATGTATTGCTATGGGTGCTTTTTAGTCCAGAACGAATTCCTGAACGAGTACG
CGAACGTCTTATTGATCCATACTTTGAGGTGTATTTCAGTGCAGCAAGTGTGTGGGAAATAGCTATAAAAGCACAGATAG
GGCGACTGAAACTTACTCTCGACTTGAATGCCATTGTTGAAGCAGCGGAGGATTCAGGATTCGAACCCCTGGCCATTCGT
ACAGAACATGCTACGAAAATTATAGATCTTCCTTTGTATCATCGAGATCCTTTTGATCGAATACTGGTCGCTCAGGCTAT
TGTCGAAAAAGCCCGGCTTTTAACTGCGGACAGCGCATTGATTAAGTACAAAAATGTGGAGCTGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8440.03 Da
• Isoelectric Point: 11.3495

>AT33066 WP_161540237.1 NZ_AP019797:380276-380506 [Rhodothermus marinus]
MRVVNIREARVHLSRLIAAVEQGEEVIIMRAGRPVARLVPLRKPRKLGVLAGRMTIPEDFDAPLPEKILSAFEGNA

Nucleotide

• Length: 231 bp

>AT33066 NZ_AP019797:380276-380506 [Rhodothermus marinus]
ATGCGGGTTGTTAACATCAGAGAAGCCCGGGTGCATCTTTCTCGGTTGATCGCTGCGGTGGAGCAGGGAGAGGAGGTCAT
CATTATGCGGGCGGGGCGTCCGGTCGCCAGACTGGTACCCCTGCGGAAGCCGCGAAAGCTGGGTGTGCTGGCGGGACGCA
TGACTATTCCCGAAGATTTCGACGCACCGCTGCCGGAAAAAATTCTATCCGCTTTTGAAGGAAATGCCTGA