Detailed information of TA system TA033063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 140945..141666
• Replicon: plasmid pAA229
• Accession: NZ_AP019795
• Organism: Thermus thermophilus strain AA2-29

Toxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FPG73_RS11285
• Protein ID: WP_014511247.1
• Coordinates: 140945..141361 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPG73_RS11290
• Protein ID: WP_143587270.1
• Coordinates: 141358..141666 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPG73_RS11265	137318..137851	+	534	Protein_161	YbgA family protein	-
FPG73_RS11270	137882..138274	-	393	WP_011174492.1	nucleotidyltransferase domain-containing protein	-
FPG73_RS11275	138284..139282	-	999	WP_143587267.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
FPG73_RS11280	139279..140847	-	1569	WP_143587269.1	phytoene desaturase	-
FPG73_RS11285	140945..141361	-	417	WP_014511247.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPG73_RS11290	141358..141666	-	309	WP_143587270.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPG73_RS11295	141672..142694	-	1023	WP_143587272.1	glycosyltransferase	-
FPG73_RS11300	142691..143368	-	678	WP_143587274.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
FPG73_RS11305	143373..144071	-	699	WP_143587276.1	lycopene cyclase domain-containing protein	-
FPG73_RS11310	144068..145237	-	1170	WP_143587278.1	cytochrome P450	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..215278	215278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-129)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (20-83)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15241.82 Da
• Isoelectric Point: 10.4393

>T33063 WP_014511247.1 NZ_AP019795:c141361-140945 [Thermus thermophilus]
MRYLLDTNVVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAE
VMELWGELTGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 417 bp

>T33063 NZ_AP019795:c141361-140945 [Thermus thermophilus]
CTGAGGTACCTGCTGGACACCAACGTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCG
CCACCTTAAAGTGGGGGAGGCGTACCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGG
CCCGAAGGGCCATCCTCAGCCGATGGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAG
GTGATGGAGCTCTGGGGCGAGCTCACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGC
GGCCACGGCCCTGCGGCACGGCCTGATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGA
ACCCCTGGGAAGGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11542.20 Da
• Isoelectric Point: 4.9544

>AT33063 WP_143587270.1 NZ_AP019795:c141666-141358 [Thermus thermophilus]
VASELDGPVFSVYPEGVKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKT
LPNEEVEALFQRQKTGFREVDF

Nucleotide

• Length: 309 bp

>AT33063 NZ_AP019795:c141666-141358 [Thermus thermophilus]
GTGGCCTCTGAACTAGACGGACCGGTCTTTTCCGTGTACCCTGAGGGGGTGAAGGCCATCTGGCAACTCCAAGAGGCCAA
AGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCAGCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGG
TGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCTCGGTCCTCGAGGCCCTAAGACCCCCCAAGACC
CTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGCTTCCGGGAGGTGGACTTCTGA