Detailed information of TA system TA033061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 170359..171080
• Replicon: plasmid pAA220
• Accession: NZ_AP019793
• Organism: Thermus thermophilus strain AA2-20

Toxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AA2Tth_RS11450
• Protein ID: WP_014511247.1
• Coordinates: 170359..170775 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AA2Tth_RS11455
• Protein ID: WP_143587270.1
• Coordinates: 170772..171080 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA2Tth_RS11430	166732..167265	+	534	Protein_189	YbgA family protein	-
AA2Tth_RS11435	167296..167688	-	393	WP_011174492.1	nucleotidyltransferase domain-containing protein	-
AA2Tth_RS11440	167698..168696	-	999	WP_143587267.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
AA2Tth_RS11445	168693..170261	-	1569	WP_143587269.1	phytoene desaturase	-
AA2Tth_RS11450	170359..170775	-	417	WP_014511247.1	type II toxin-antitoxin system VapC family toxin	Toxin
AA2Tth_RS11455	170772..171080	-	309	WP_143587270.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AA2Tth_RS11460	171086..172108	-	1023	WP_143587272.1	glycosyltransferase	-
AA2Tth_RS11465	172105..172782	-	678	WP_143587274.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
AA2Tth_RS11470	172787..173485	-	699	WP_143587276.1	lycopene cyclase domain-containing protein	-
AA2Tth_RS11475	173482..174651	-	1170	WP_143587278.1	cytochrome P450	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..219630	219630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-129)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (20-83)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15241.82 Da
• Isoelectric Point: 10.4393

>T33061 WP_014511247.1 NZ_AP019793:c170775-170359 [Thermus thermophilus]
MRYLLDTNVVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAE
VMELWGELTGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 417 bp

>T33061 NZ_AP019793:c170775-170359 [Thermus thermophilus]
CTGAGGTACCTGCTGGACACCAACGTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCG
CCACCTTAAAGTGGGGGAGGCGTACCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGG
CCCGAAGGGCCATCCTCAGCCGATGGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAG
GTGATGGAGCTCTGGGGCGAGCTCACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGC
GGCCACGGCCCTGCGGCACGGCCTGATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGA
ACCCCTGGGAAGGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11542.20 Da
• Isoelectric Point: 4.9544

>AT33061 WP_143587270.1 NZ_AP019793:c171080-170772 [Thermus thermophilus]
VASELDGPVFSVYPEGVKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKT
LPNEEVEALFQRQKTGFREVDF

Nucleotide

• Length: 309 bp

>AT33061 NZ_AP019793:c171080-170772 [Thermus thermophilus]
GTGGCCTCTGAACTAGACGGACCGGTCTTTTCCGTGTACCCTGAGGGGGTGAAGGCCATCTGGCAACTCCAAGAGGCCAA
AGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCAGCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGG
TGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCTCGGTCCTCGAGGCCCTAAGACCCCCCAAGACC
CTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGCTTCCGGGAGGTGGACTTCTGA