Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 88172..89682 Replicon plasmid pRS11DPr2
Accession NZ_AP019785
Organism Methylomagnum ishizawai strain RS11D-Pr

Toxin (Protein)


Gene name rlegT Uniprot ID -
Locus tag K5658_RS22145 Protein ID WP_221067259.1
Coordinates 88172..89086 (-) Length 305 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID -
Locus tag K5658_RS22150 Protein ID WP_221067260.1
Coordinates 89083..89682 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5658_RS24045 (MishRS11D_44080) 83450..84280 + 831 WP_221067254.1 TPM domain-containing protein -
K5658_RS22125 (MishRS11D_44090) 84280..84789 + 510 WP_221067255.1 TPM domain-containing protein -
K5658_RS22130 (MishRS11D_44100) 85151..86206 - 1056 WP_221067256.1 transposase -
K5658_RS22135 (MishRS11D_44110) 86305..86631 + 327 WP_221067257.1 hypothetical protein -
K5658_RS22140 (MishRS11D_44120) 86828..87853 - 1026 WP_221067258.1 replication protein -
K5658_RS22145 (MishRS11D_44130) 88172..89086 - 915 WP_221067259.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
K5658_RS22150 (MishRS11D_44140) 89083..89682 - 600 WP_221067260.1 AbiEi antitoxin N-terminal domain-containing protein Antitoxin
K5658_RS22155 (MishRS11D_44150) 90171..90998 + 828 WP_221067261.1 IS5 family transposase -
K5658_RS22160 (MishRS11D_44160) 91023..91619 - 597 WP_221067262.1 type 1 glutamine amidotransferase domain-containing protein -
K5658_RS22165 (MishRS11D_44170) 91805..92539 - 735 WP_246628692.1 ISAzo13 family transposase -
K5658_RS22170 (MishRS11D_44180) 92640..92915 + 276 WP_221067263.1 acyl carrier protein -
K5658_RS22175 (MishRS11D_44190) 93119..94183 + 1065 WP_221067264.1 TauD/TfdA family dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..133705 133705


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(36-269)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(6-51)


Sequences

Toxin        


Download         Length: 305 a.a.        Molecular weight: 33891.90 Da        Isoelectric Point: 7.9146

>T33059 WP_221067259.1 NZ_AP019785:c89086-88172 [Methylomagnum ishizawai]
MSERNLAASVRARLLNRARETRQDFNLVLTRYALERLLYRLSVSPHADQFLLKGALLFDLWFDIPHRPTRDADFLGFGPA
ELPSLEAVFQEVCVVDADDGMMFRPDTVRAAEIRKEANYAGVRVTLLGLIDGARCPMQIDIGFGDAVTPGPEDAQYPVML
PEFTAPRLRVYPRYTVVAEKVEALASLGIANSRMKDYFDLWILSRHTGFDGDTLRRALRATFDRRKTGLPPGAPLGLTDG
FARDAQKRTQWRAFLGKNRLEAPPLEDVVASLRDFLLPVIAAASADAHPPRHWPAGGPWTPVVG

Download         Length: 915 bp

>T33059 NZ_AP019785:c89086-88172 [Methylomagnum ishizawai]
ATGAGCGAACGGAACCTAGCGGCATCCGTGCGGGCACGCCTGCTCAACCGCGCCCGCGAAACCCGGCAGGACTTCAATCT
GGTCCTGACCCGCTACGCGCTGGAGCGCCTGCTGTACCGGCTCAGTGTTTCCCCCCACGCCGACCAGTTCCTGCTCAAGG
GTGCCTTGCTGTTCGACCTGTGGTTCGACATCCCGCACCGCCCGACCAGGGACGCCGACTTCCTCGGATTCGGCCCGGCC
GAGTTGCCAAGCCTCGAAGCCGTCTTCCAGGAAGTCTGCGTGGTCGACGCCGACGACGGGATGATGTTCCGGCCGGACAC
CGTCCGGGCGGCGGAGATCCGGAAGGAGGCCAACTACGCCGGCGTGCGCGTCACCTTGCTCGGGCTGATCGACGGCGCGC
GCTGCCCCATGCAGATCGACATCGGTTTCGGCGATGCCGTGACGCCGGGGCCGGAGGACGCCCAGTACCCCGTGATGCTC
CCGGAGTTCACGGCACCGAGGCTGCGCGTCTATCCGCGCTATACCGTGGTGGCTGAAAAGGTGGAGGCATTGGCCTCGCT
CGGGATCGCCAACAGCCGGATGAAGGATTATTTCGACCTCTGGATCCTGTCGCGCCACACCGGATTCGACGGCGACACCC
TGCGCCGCGCCCTCCGGGCAACCTTCGATCGCCGCAAGACGGGGTTGCCCCCGGGCGCCCCCTTGGGCCTGACCGACGGT
TTTGCGCGGGACGCGCAAAAGCGGACGCAGTGGCGGGCGTTCCTGGGAAAGAACAGGCTGGAAGCGCCGCCCCTGGAGGA
CGTCGTGGCATCGCTCCGGGATTTCTTGCTTCCGGTCATCGCGGCGGCGAGCGCCGACGCCCACCCCCCGCGACACTGGC
CGGCTGGCGGGCCCTGGACTCCGGTGGTGGGTTGA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22098.66 Da        Isoelectric Point: 11.0204

>AT33059 WP_221067260.1 NZ_AP019785:c89682-89083 [Methylomagnum ishizawai]
MQSSHQTILDLADERGLIRPRDLAALGLPSVALTRLVRQGRLARVGRGLYGRPDRAVSEHGTLAGVARKHPQGIVCLLSA
LRVHDLTTQSPSEVWLAIPNKARAPRMDYPPLRIVRFSGAALTEGIEEHVIDGVPVRVTNVARTVADCFKFRNKIGLDVA
LEALREAWRARRSSMDELWRFAAPCRVANVMRPYLESLP

Download         Length: 600 bp

>AT33059 NZ_AP019785:c89682-89083 [Methylomagnum ishizawai]
ATGCAAAGCTCGCATCAGACCATTCTGGACCTGGCCGATGAGCGCGGCCTGATCCGCCCGCGCGACCTCGCTGCGCTTGG
GCTGCCCAGTGTCGCCCTCACCCGGCTGGTCCGGCAGGGTCGGCTCGCCCGGGTGGGGCGCGGCCTGTATGGCCGTCCCG
ACCGCGCCGTGTCGGAGCATGGCACGCTGGCCGGGGTCGCGCGCAAACACCCGCAAGGCATCGTCTGCCTGCTGTCGGCG
CTGCGCGTGCACGACCTCACCACGCAGTCACCGTCCGAGGTCTGGTTGGCCATCCCCAACAAGGCGCGCGCGCCGAGGAT
GGACTATCCCCCGCTGCGCATCGTTCGCTTCTCCGGCGCCGCGCTGACCGAAGGGATCGAAGAACACGTCATCGACGGCG
TGCCGGTGCGGGTGACCAACGTCGCCCGGACGGTGGCCGACTGCTTCAAGTTCCGCAACAAGATCGGTCTCGATGTCGCG
CTGGAGGCGCTACGGGAGGCGTGGCGGGCCAGGCGGTCCAGCATGGACGAACTGTGGCGGTTCGCGGCGCCGTGCAGGGT
GGCCAATGTGATGCGCCCCTACCTGGAAAGCCTGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References