Detailed information of TA system TA033054

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 2298951..2299797
• Replicon: chromosome
• Accession: NZ_AP019782
• Organism: Methylogaea oryzae strain E10

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: A0A8D4VS42
• Locus tag: K5607_RS10030
• Protein ID: WP_054773129.1
• Coordinates: 2298951..2299412 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: A0A8D5AIH3
• Locus tag: K5607_RS10035
• Protein ID: WP_054773128.1
• Coordinates: 2299402..2299797 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5607_RS10010 (MoryE10_19520)	2294205..2294894	+	690	WP_221046936.1	OmpA family protein	-
K5607_RS10015 (MoryE10_19530)	2294959..2296578	-	1620	WP_054773130.1	dipeptide ABC transporter ATP-binding protein	-
K5607_RS10020 (MoryE10_19540)	2296583..2297974	-	1392	WP_246599018.1	ABC transporter permease	-
K5607_RS10025 (MoryE10_19550)	2297977..2298954	-	978	WP_221046937.1	ABC transporter permease	-
K5607_RS10030 (MoryE10_19560)	2298951..2299412	-	462	WP_054773129.1	DUF86 domain-containing protein	Toxin
K5607_RS10035 (MoryE10_19570)	2299402..2299797	-	396	WP_054773128.1	nucleotidyltransferase domain-containing protein	Antitoxin
K5607_RS10040 (MoryE10_19580)	2299832..2301979	-	2148	WP_246598820.1	ABC transporter substrate-binding protein	-
K5607_RS10045 (MoryE10_19590)	2302143..2302931	+	789	WP_054773127.1	enoyl-ACP reductase FabI	-
K5607_RS10050 (MoryE10_19600)	2303073..2303486	+	414	WP_054773126.1	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-
K5607_RS10055 (MoryE10_19610)	2303528..2304700	+	1173	WP_221046938.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (19-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (6-94)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16822.01 Da
• Isoelectric Point: 6.2361

>T33054 WP_054773129.1 NZ_AP019782:c2299412-2298951 [Methylogaea oryzae]
MADDVLINKAATIERSVGRAREEYQRDPAGFGADFTRQDAAVLNIQRACEAALDMGQHLIRREKLGVPQSARDVFALLAQ
GGWIEPALADGLKRMVGFRNIAVHDYQSLQLPITVNIIEKHLDEFLRYSQTLLQRDAATATASGASDTIGNRS

Nucleotide

• Length: 462 bp

>T33054 NZ_AP019782:c2299412-2298951 [Methylogaea oryzae]
ATGGCCGATGACGTGCTCATCAATAAGGCCGCCACTATCGAGCGGAGCGTCGGACGCGCCCGCGAGGAATACCAGCGGGA
TCCGGCCGGCTTCGGCGCGGATTTCACCCGCCAGGACGCGGCCGTCTTGAACATCCAGCGCGCTTGCGAGGCGGCGTTGG
ACATGGGCCAGCACCTGATACGCCGCGAAAAGCTGGGGGTGCCGCAAAGCGCCCGCGACGTGTTCGCGTTACTGGCGCAG
GGCGGCTGGATCGAGCCTGCGCTGGCCGACGGGCTCAAGCGCATGGTGGGGTTTCGCAATATCGCTGTGCACGATTACCA
GAGCTTGCAACTGCCTATCACCGTGAACATCATCGAAAAGCACCTCGATGAATTCCTGCGCTACAGCCAGACGCTGCTGC
AACGCGATGCGGCCACGGCCACGGCATCGGGAGCCAGCGATACAATTGGAAATCGCTCATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14018.08 Da
• Isoelectric Point: 4.4299

>AT33054 WP_054773128.1 NZ_AP019782:c2299797-2299402 [Methylogaea oryzae]
MNRAAIIEILQARLPNLQAIYAFGSRIAGTAQAGSDLDLAVLVAGYADPLVLWELAGDLAELAGCPVDLLDLRVASTVMQ
YQIVTTGQRWWARDAQAALYEAAILSDKTELDTARAGLLADIAKSGRVYGR

Nucleotide

• Length: 396 bp

>AT33054 NZ_AP019782:c2299797-2299402 [Methylogaea oryzae]
ATGAACCGCGCAGCGATCATTGAAATCCTGCAAGCCCGCCTGCCGAATTTGCAGGCCATCTATGCCTTTGGCAGCCGGAT
TGCGGGTACGGCACAAGCCGGCAGCGACCTCGATCTCGCCGTGCTGGTGGCGGGTTACGCCGACCCGCTGGTTCTGTGGG
AGTTGGCGGGCGATCTGGCGGAGCTGGCCGGCTGCCCCGTGGATTTGCTCGACCTGCGCGTCGCTTCCACCGTGATGCAG
TACCAGATTGTCACCACCGGCCAACGCTGGTGGGCTAGAGACGCGCAAGCGGCTTTGTACGAGGCCGCCATCCTCAGCGA
TAAGACCGAGCTGGATACGGCTCGCGCCGGGCTGCTGGCCGATATCGCAAAATCCGGGAGGGTTTATGGCCGATGA