Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-UPF0150
Location 1571876..1572319 Replicon chromosome
Accession NZ_AP019780
Organism Methanosarcina mazei strain TMA

Toxin (Protein)


Gene name hicA Uniprot ID A0A0F8R3S9
Locus tag MmazTMA_RS06705 Protein ID WP_048037760.1
Coordinates 1571876..1572100 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0F8BVJ6
Locus tag MmazTMA_RS06710 Protein ID WP_048037761.1
Coordinates 1572101..1572319 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MmazTMA_RS06690 (MmazTMA_13500) 1567193..1567495 + 303 WP_048037757.1 DUF86 domain-containing protein -
MmazTMA_RS06695 (MmazTMA_13510) 1567765..1570362 - 2598 WP_048037759.1 UvrD-helicase domain-containing protein -
MmazTMA_RS06700 (MmazTMA_13520) 1570864..1571796 + 933 WP_050035795.1 Fic family protein -
MmazTMA_RS06705 (MmazTMA_13530) 1571876..1572100 - 225 WP_048037760.1 type II toxin-antitoxin system HicA family toxin Toxin
MmazTMA_RS06710 (MmazTMA_13540) 1572101..1572319 - 219 WP_048037761.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MmazTMA_RS06715 (MmazTMA_13550) 1572702..1573007 + 306 WP_048037755.1 nucleotidyltransferase -
MmazTMA_RS06720 (MmazTMA_13560) 1573062..1573364 + 303 WP_048037757.1 DUF86 domain-containing protein -
MmazTMA_RS06725 (MmazTMA_13570) 1573899..1574246 + 348 WP_011031982.1 hypothetical protein -
MmazTMA_RS06730 (MmazTMA_13580) 1574594..1575139 + 546 WP_011031981.1 DUF6398 domain-containing protein -
MmazTMA_RS19105 (MmazTMA_13590) 1575544..1575693 + 150 WP_226987654.1 hypothetical protein -
MmazTMA_RS06740 (MmazTMA_13600) 1576000..1576359 - 360 WP_011031980.1 DUF6516 family protein -
MmazTMA_RS06745 (MmazTMA_13610) 1576356..1576676 - 321 WP_226987653.1 hypothetical protein -
MmazTMA_RS06750 (MmazTMA_13620) 1576884..1577108 - 225 WP_011031978.1 type II toxin-antitoxin system HicA family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-64)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-56)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8103.66 Da        Isoelectric Point: 11.0007

>T33051 WP_048037760.1 NZ_AP019780:c1572100-1571876 [Methanosarcina mazei]
MFGLPVISGMQAIKAFSKAGWLPHRQVGSHVVLRKEGSKVTLTVPNHKELKPGLLRNLIKASGLTVEEFEALLK

Download         Length: 225 bp

>T33051 NZ_AP019780:c1572100-1571876 [Methanosarcina mazei]
ATGTTTGGACTTCCTGTAATTTCCGGAATGCAGGCAATCAAAGCTTTTTCTAAAGCTGGCTGGCTCCCACACCGGCAGGT
AGGAAGCCACGTTGTCCTGAGAAAGGAAGGATCAAAAGTTACGCTTACTGTCCCGAATCACAAAGAACTGAAGCCGGGTC
TTTTAAGAAACTTGATTAAAGCTTCCGGGCTCACGGTAGAAGAATTCGAAGCTCTTTTAAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7735.73 Da        Isoelectric Point: 3.7317

>AT33051 WP_048037761.1 NZ_AP019780:c1572319-1572101 [Methanosarcina mazei]
MRFKIILEEDEDVGGFIASCPGLPGCFSQGDTVEEAIENIKEAIQACLESLAEDDLQECIGKSSCRVVDVVA

Download         Length: 219 bp

>AT33051 NZ_AP019780:c1572319-1572101 [Methanosarcina mazei]
ATGCGGTTCAAAATTATCCTTGAGGAAGACGAAGATGTAGGGGGATTTATTGCCAGCTGCCCCGGTTTGCCAGGCTGTTT
TTCACAGGGAGATACTGTAGAAGAAGCTATCGAAAACATTAAAGAAGCGATTCAGGCTTGCCTGGAATCCCTTGCAGAAG
ACGACTTGCAGGAATGCATTGGAAAATCCTCCTGCAGAGTTGTTGATGTGGTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F8R3S9

Antitoxin

Source ID Structure
AlphaFold DB A0A0F8BVJ6

References