Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 172140..172726 Replicon plasmid pFW12EY
Accession NZ_AP019778
Organism Methylomonas koyamae strain Fw12E-Y

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag MKFW12EY_RS22590 Protein ID WP_054758393.1
Coordinates 172140..172478 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag MKFW12EY_RS22595 Protein ID WP_054758391.1
Coordinates 172478..172726 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKFW12EY_RS22555 (MKFW12EY_44980) 167921..168130 + 210 WP_221054660.1 hypothetical protein -
MKFW12EY_RS22560 (MKFW12EY_44990) 168101..168391 - 291 WP_157199139.1 hypothetical protein -
MKFW12EY_RS22565 (MKFW12EY_45000) 168502..168738 + 237 WP_054758403.1 hypothetical protein -
MKFW12EY_RS22570 (MKFW12EY_45010) 168753..168983 - 231 WP_054758400.1 hypothetical protein -
MKFW12EY_RS22575 (MKFW12EY_45020) 169291..170289 - 999 WP_221054661.1 YqaJ viral recombinase family protein -
MKFW12EY_RS22580 (MKFW12EY_45030) 170286..170633 - 348 WP_054758397.1 hypothetical protein -
MKFW12EY_RS22585 (MKFW12EY_45040) 170724..171785 - 1062 WP_245006533.1 phage recombination protein Bet -
MKFW12EY_RS22590 (MKFW12EY_45050) 172140..172478 - 339 WP_054758393.1 endoribonuclease MazF Toxin
MKFW12EY_RS22595 (MKFW12EY_45060) 172478..172726 - 249 WP_054758391.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MKFW12EY_RS22600 (MKFW12EY_45070) 173065..173970 + 906 WP_082409595.1 DNA-binding protein -
MKFW12EY_RS22605 174252..174386 - 135 Protein_142 transposase -
MKFW12EY_RS22610 (MKFW12EY_45080) 174611..175201 + 591 WP_221054662.1 cohesin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..241446 241446


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-111)

Antitoxin

SpoVT-AbrB domain

(6-48)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12094.08 Da        Isoelectric Point: 6.9777

>T33050 WP_054758393.1 NZ_AP019778:c172478-172140 [Methylomonas koyamae]
MAVSYVPERGDLVWIAFDPQAGHEQAGRRPGLVVTPRTYNVKAGLALICPVTSRVKGYPFEVPLPVGDRVSGVILADQIR
SLDWAARQAEKISMVDGAVLAEVLAKIEALIQ

Download         Length: 339 bp

>T33050 NZ_AP019778:c172478-172140 [Methylomonas koyamae]
ATGGCTGTATCGTACGTACCTGAGCGCGGGGATTTGGTGTGGATTGCGTTCGATCCTCAAGCAGGCCACGAACAAGCGGG
GCGCCGCCCCGGCCTGGTCGTTACGCCACGCACTTATAACGTCAAAGCCGGGTTGGCGTTGATTTGCCCTGTAACAAGTC
GGGTTAAAGGATATCCGTTTGAGGTACCGTTACCGGTCGGCGACCGTGTGAGTGGTGTGATATTGGCGGATCAGATTCGC
AGTCTGGATTGGGCGGCGCGTCAGGCCGAAAAGATATCTATGGTGGACGGGGCGGTATTGGCCGAAGTCTTGGCCAAAAT
CGAGGCTCTTATTCAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8929.21 Da        Isoelectric Point: 4.5388

>AT33050 WP_054758391.1 NZ_AP019778:c172726-172478 [Methylomonas koyamae]
MQTEIKKWGNSAVIRLPAAMLAQLKLEVGSPVELTADDAGLRIVPTTAKPVFKLEELLAGMTDDNRHDCIDWGPDVGREG
NN

Download         Length: 249 bp

>AT33050 NZ_AP019778:c172726-172478 [Methylomonas koyamae]
ATGCAAACTGAAATTAAAAAATGGGGTAATAGCGCCGTGATTCGATTGCCGGCGGCGATGCTGGCACAGTTAAAGCTGGA
AGTCGGTTCGCCGGTCGAGCTGACAGCGGACGATGCCGGGCTACGGATTGTACCGACCACGGCCAAACCTGTTTTTAAGT
TGGAGGAATTACTGGCTGGCATGACTGACGATAATCGGCATGATTGCATTGACTGGGGGCCGGATGTTGGCCGAGAGGGT
AACAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References