Detailed information of TA system TA033049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 125427..126021
• Replicon: plasmid pFW12EY
• Accession: NZ_AP019778
• Organism: Methylomonas koyamae strain Fw12E-Y

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: MKFW12EY_RS22355
• Protein ID: WP_054758459.1
• Coordinates: 125427..125717 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: MKFW12EY_RS22360
• Protein ID: WP_221054647.1
• Coordinates: 125728..126021 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKFW12EY_RS22345 (MKFW12EY_44560)	122101..124434	+	2334	WP_245006529.1	DEAD/DEAH box helicase family protein	-
MKFW12EY_RS22350 (MKFW12EY_44570)	124607..125212	-	606	Protein_90	AAA family ATPase	-
MKFW12EY_RS22355 (MKFW12EY_44580)	125427..125717	+	291	WP_054758459.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MKFW12EY_RS22360 (MKFW12EY_44590)	125728..126021	+	294	WP_221054647.1	putative addiction module antidote protein	Antitoxin
MKFW12EY_RS22365 (MKFW12EY_44600)	126272..126727	+	456	WP_157199147.1	hypothetical protein	-
MKFW12EY_RS22370 (MKFW12EY_44610)	126957..128144	+	1188	WP_245006530.1	TolC family protein	-
MKFW12EY_RS22375 (MKFW12EY_44620)	128161..129636	+	1476	WP_054758455.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..241446	241446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10690.25 Da
• Isoelectric Point: 9.8609

>T33049 WP_054758459.1 NZ_AP019778:125427-125717 [Methylomonas koyamae]
MVDIKKTNVYARWLDDLRDIRARARVLARVERLVAGNPGDVKSVGEGVLEMRIDYGPGYRVYFTQRGNEIVILLAGGDKS
TQDADIKTAQGLARNL

Nucleotide

• Length: 291 bp

>T33049 NZ_AP019778:125427-125717 [Methylomonas koyamae]
ATGGTCGATATAAAGAAAACCAATGTTTATGCCCGATGGCTGGACGATCTTCGCGATATTCGTGCTCGCGCAAGAGTTTT
GGCCAGGGTCGAACGTCTGGTTGCTGGCAATCCGGGTGACGTTAAGTCGGTTGGGGAAGGTGTATTAGAAATGCGAATCG
ACTATGGCCCCGGTTATCGGGTGTATTTCACGCAACGCGGCAACGAAATCGTCATTTTGTTGGCTGGTGGCGATAAAAGC
ACTCAGGATGCCGATATTAAAACCGCCCAAGGTCTGGCCCGTAACCTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10588.99 Da
• Isoelectric Point: 5.1152

>AT33049 WP_221054647.1 NZ_AP019778:125728-126021 [Methylomonas koyamae]
MTKTIISRYDVAEHLRTTEEMAAYLDACIEEADGDAAFIAKALGDIARARGMSQIARDTGLSRESLYRSLDGEHIPSFDT
ILKVTKALGIHLHADPA

Nucleotide

• Length: 294 bp

>AT33049 NZ_AP019778:125728-126021 [Methylomonas koyamae]
ATGACAAAAACTATCATCAGCCGCTACGATGTCGCCGAGCACTTGAGAACCACCGAAGAAATGGCGGCCTATCTGGATGC
CTGCATTGAGGAGGCCGATGGCGATGCGGCCTTCATAGCCAAGGCACTTGGCGATATTGCCCGTGCACGCGGCATGTCGC
AGATTGCCCGAGATACTGGGCTTTCCCGCGAAAGCCTGTATCGATCCCTGGACGGCGAGCACATCCCCAGTTTCGATACG
ATCCTCAAGGTAACGAAGGCGTTAGGCATTCATCTACATGCCGATCCGGCCTGA