Detailed information of TA system TA033048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 20927..21563
• Replicon: plasmid pFW12EY
• Accession: NZ_AP019778
• Organism: Methylomonas koyamae strain Fw12E-Y

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MKFW12EY_RS21955
• Protein ID: WP_054759933.1
• Coordinates: 21147..21563 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A172UFQ6
• Locus tag: MKFW12EY_RS21950
• Protein ID: WP_064023836.1
• Coordinates: 20927..21160 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKFW12EY_RS21935 (MKFW12EY_43720)	16773..18521	+	1749	WP_221054679.1	AAA family ATPase	-
MKFW12EY_RS21940 (MKFW12EY_43730)	18494..20242	+	1749	WP_054759919.1	ATP-dependent helicase	-
MKFW12EY_RS21945 (MKFW12EY_43740)	20533..20678	-	146	Protein_10	cold shock domain-containing protein	-
MKFW12EY_RS21950 (MKFW12EY_43750)	20927..21160	+	234	WP_064023836.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MKFW12EY_RS21955 (MKFW12EY_43760)	21147..21563	+	417	WP_054759933.1	PIN domain-containing protein	Toxin
MKFW12EY_RS21960 (MKFW12EY_43770)	21759..22796	-	1038	WP_157199241.1	hypothetical protein	-
MKFW12EY_RS21965 (MKFW12EY_43780)	22768..24180	-	1413	WP_221054680.1	MBOAT family O-acyltransferase	-
MKFW12EY_RS21970 (MKFW12EY_43790)	24197..24565	-	369	WP_054759910.1	hypothetical protein	-
MKFW12EY_RS21975 (MKFW12EY_43800)	24562..25053	-	492	WP_064023679.1	thermonuclease family protein	-
MKFW12EY_RS21980 (MKFW12EY_43810)	25068..25700	-	633	WP_054759906.1	DUF4400 domain-containing protein	-
MKFW12EY_RS21985 (MKFW12EY_43820)	25703..26101	-	399	WP_157199239.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..241446	241446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15110.38 Da
• Isoelectric Point: 4.3875

>T33048 WP_054759933.1 NZ_AP019778:21147-21563 [Methylomonas koyamae]
MKTAKPFLDTNVLLYLLSEDNQKADRAEEIIAFGGIINVQVLNEFASVASRKLKMTFVEIRETLSIIRSICETQALSVSI
HEQGLAIAEQFGFSLYDSMIVSAALDAGCSILYSEDMQHGQAIGSLVINNPFLPSLPN

Nucleotide

• Length: 417 bp

>T33048 NZ_AP019778:21147-21563 [Methylomonas koyamae]
ATGAAAACCGCTAAGCCGTTTCTCGATACCAACGTCTTGCTGTATTTACTGTCTGAGGACAATCAAAAAGCAGACCGCGC
CGAAGAGATTATCGCCTTCGGCGGCATAATCAATGTGCAGGTGCTGAATGAATTTGCGTCGGTAGCGTCGCGCAAGCTGA
AGATGACGTTTGTCGAAATCCGCGAAACCTTGTCGATTATCCGAAGTATCTGTGAAACTCAAGCTTTGAGCGTCAGCATC
CACGAACAAGGTTTGGCGATTGCCGAACAATTTGGTTTTTCGTTGTATGACAGCATGATTGTCAGCGCGGCGCTGGACGC
CGGGTGCTCAATATTGTATTCGGAAGACATGCAACATGGTCAAGCTATTGGCTCGTTAGTCATTAACAATCCGTTTTTAC
CTTCTCTTCCTAACTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8756.09 Da
• Isoelectric Point: 7.2054

>AT33048 WP_064023836.1 NZ_AP019778:20927-21160 [Methylomonas koyamae]
MQVAKWGNSLAIRLPATVVEALELKEGDDIEVHIAGERVFELEKKPSAKTLLAKLHQFRGRLPADFKFDRLEANENR

Nucleotide

• Length: 234 bp

>AT33048 NZ_AP019778:20927-21160 [Methylomonas koyamae]
ATGCAAGTCGCAAAATGGGGTAATAGTTTAGCTATTCGTTTGCCCGCCACGGTGGTAGAAGCGTTAGAGTTAAAGGAAGG
CGATGACATTGAAGTACATATCGCCGGCGAACGGGTGTTTGAACTAGAGAAAAAACCTTCGGCAAAGACGTTGTTAGCCA
AATTGCATCAATTTCGTGGGCGCCTACCCGCTGATTTTAAGTTTGACCGCCTGGAAGCGAATGAAAACCGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A172UFQ6