Detailed information of TA system TA033047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 4231524..4232160
• Replicon: chromosome
• Accession: NZ_AP019777
• Organism: Methylomonas koyamae strain Fw12E-Y

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MKFW12EY_RS19075
• Protein ID: WP_054762077.1
• Coordinates: 4231744..4232160 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MKFW12EY_RS19070
• Protein ID: WP_054762089.1
• Coordinates: 4231524..4231757 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKFW12EY_RS19050 (MKFW12EY_37920)	4228034..4229071	-	1038	WP_221053574.1	hydrogenase expression/formation protein HypE	-
MKFW12EY_RS19055 (MKFW12EY_37930)	4229137..4229364	-	228	WP_054762071.1	type II toxin-antitoxin system HicA family toxin	-
MKFW12EY_RS19060 (MKFW12EY_37940)	4229367..4229576	-	210	WP_054762073.1	type II toxin-antitoxin system HicB family antitoxin	-
MKFW12EY_RS19065 (MKFW12EY_37950)	4229895..4231187	-	1293	WP_054762075.1	hypothetical protein	-
MKFW12EY_RS19070 (MKFW12EY_37960)	4231524..4231757	+	234	WP_054762089.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MKFW12EY_RS19075 (MKFW12EY_37970)	4231744..4232160	+	417	WP_054762077.1	PIN domain-containing protein	Toxin
MKFW12EY_RS19080 (MKFW12EY_37980)	4232248..4232502	+	255	WP_054762078.1	prevent-host-death protein	-
MKFW12EY_RS19085 (MKFW12EY_37990)	4232502..4232777	+	276	WP_054762080.1	plasmid stabilization protein	-
MKFW12EY_RS19090 (MKFW12EY_38000)	4232944..4233804	-	861	WP_054762081.1	hypothetical protein	-
MKFW12EY_RS19095 (MKFW12EY_38010)	4233826..4234461	-	636	WP_054762092.1	YchE family NAAT transporter	-
MKFW12EY_RS19100 (MKFW12EY_38020)	4234487..4235815	-	1329	WP_245006357.1	GTP-binding protein	-
MKFW12EY_RS19105 (MKFW12EY_38030)	4235815..4236744	-	930	WP_054763774.1	sulfate adenylyltransferase subunit CysD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-118)

Antitoxin

• SpoVT-AbrB domain (5-36)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15303.48 Da
• Isoelectric Point: 4.3759

>T33047 WP_054762077.1 NZ_AP019777:4231744-4232160 [Methylomonas koyamae]
MKTAKAFFDSNVLLYLLSADDTKANRAEALLADGGAISVQVLNEFTSVATRKFKMSYAEVREALEVVKAICQIDPLSVDI
HERGLDIAERYGFSWYDSLIVAAALVAQCECLYSEDLQHGQVVDNQLRIVNPFIDLTV

Nucleotide

• Length: 417 bp

>T33047 NZ_AP019777:4231744-4232160 [Methylomonas koyamae]
ATGAAGACCGCTAAAGCATTTTTCGACAGCAACGTGCTGCTGTATTTGCTGTCAGCCGACGATACCAAGGCAAACCGAGC
CGAAGCCTTGCTTGCCGACGGCGGCGCGATCAGCGTGCAAGTGTTGAACGAGTTTACCTCGGTCGCTACGCGCAAATTCA
AAATGTCATATGCCGAAGTCCGAGAAGCCTTAGAAGTCGTCAAAGCGATCTGTCAAATCGATCCGCTTAGCGTCGATATC
CACGAACGCGGCCTGGACATCGCCGAACGTTACGGTTTTTCCTGGTACGACAGCCTGATCGTCGCTGCGGCGTTGGTGGC
CCAATGCGAGTGCTTGTATTCGGAAGACCTGCAGCACGGCCAAGTCGTCGACAATCAATTGCGCATCGTCAATCCGTTTA
TCGACCTCACCGTTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8740.11 Da
• Isoelectric Point: 8.7461

>AT33047 WP_054762089.1 NZ_AP019777:4231524-4231757 [Methylomonas koyamae]
MQVAKWGNSLAVRLPAAVVEALQLKEGDDVEIQIAGARLFEIEKKASNLELLGRLRKFRGRMPADFKFDRLEANEDR

Nucleotide

• Length: 234 bp

>AT33047 NZ_AP019777:4231524-4231757 [Methylomonas koyamae]
ATGCAAGTCGCCAAATGGGGTAATAGTTTAGCGGTTCGTCTGCCGGCCGCCGTGGTCGAAGCCTTGCAACTGAAGGAAGG
CGACGATGTCGAGATTCAGATTGCCGGCGCACGGCTGTTCGAAATCGAAAAAAAAGCCAGTAATCTGGAACTGCTGGGCA
GGTTACGCAAATTCCGTGGCCGCATGCCAGCGGATTTCAAGTTCGATCGCTTGGAAGCGAATGAAGACCGCTAA