Detailed information of TA system TA033045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3917363..3918004
• Replicon: chromosome
• Accession: NZ_AP019777
• Organism: Methylomonas koyamae strain Fw12E-Y

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MKFW12EY_RS17570
• Protein ID: WP_054762000.1
• Coordinates: 3917603..3918004 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A291IN90
• Locus tag: MKFW12EY_RS17565
• Protein ID: WP_054761998.1
• Coordinates: 3917363..3917596 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKFW12EY_RS17545 (MKFW12EY_35050)	3912541..3913494	-	954	WP_221053478.1	CFI-box-CTERM domain-containing protein	-
MKFW12EY_RS17550 (MKFW12EY_35060)	3913740..3915557	-	1818	WP_221053479.1	ABC transporter ATP-binding protein	-
MKFW12EY_RS17555 (MKFW12EY_35070)	3916410..3916676	+	267	WP_054761995.1	hypothetical protein	-
MKFW12EY_RS17560 (MKFW12EY_35080)	3916676..3917050	+	375	WP_221053480.1	type II toxin-antitoxin system VapC family toxin	-
MKFW12EY_RS17565 (MKFW12EY_35090)	3917363..3917596	+	234	WP_054761998.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MKFW12EY_RS17570 (MKFW12EY_35100)	3917603..3918004	+	402	WP_054762000.1	type II toxin-antitoxin system VapC family toxin	Toxin
MKFW12EY_RS17575 (MKFW12EY_35110)	3918495..3919520	+	1026	WP_221053481.1	hypothetical protein	-
MKFW12EY_RS17580 (MKFW12EY_35120)	3919522..3920136	+	615	WP_054762002.1	hypothetical protein	-
MKFW12EY_RS17585 (MKFW12EY_35130)	3920803..3921210	-	408	WP_054762005.1	type II toxin-antitoxin system VapC family toxin	-
MKFW12EY_RS17590 (MKFW12EY_35140)	3921215..3921472	-	258	WP_054762008.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MKFW12EY_RS17595 (MKFW12EY_35150)	3921925..3922806	+	882	WP_221053482.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14952.37 Da
• Isoelectric Point: 7.5180

>T33045 WP_054762000.1 NZ_AP019777:3917603-3918004 [Methylomonas koyamae]
MLKYLLDTNIVIYVIKQRPIEVLATFNRHHGRMAISAITLAELIHGAEKSQFPARNLATVEDFCSRLQVLPYTDNAAMHY
GGIRAALEKIGQPIGVNDLHIAAHARSHGLILVSNNLREFERVPGLLMENWLE

Nucleotide

• Length: 402 bp

>T33045 NZ_AP019777:3917603-3918004 [Methylomonas koyamae]
ATGTTGAAATATCTGCTCGACACCAACATCGTCATCTATGTGATCAAACAACGGCCGATCGAAGTATTGGCGACCTTCAA
CCGGCATCACGGCCGCATGGCGATTTCCGCGATTACGCTGGCTGAGCTGATCCACGGCGCCGAAAAGAGCCAATTCCCCG
CCCGCAACCTTGCTACGGTGGAAGACTTTTGCAGCCGTTTACAAGTATTGCCTTACACCGACAACGCCGCGATGCATTAC
GGCGGCATACGCGCGGCGTTGGAGAAAATCGGCCAGCCGATAGGTGTCAACGACCTGCACATCGCCGCTCATGCCCGCAG
CCATGGCTTGATATTGGTCAGCAATAATCTGCGAGAATTCGAGCGAGTGCCGGGGTTGTTGATGGAGAATTGGTTGGAGT
GA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8618.53 Da
• Isoelectric Point: 4.4883

>AT33045 WP_054761998.1 NZ_AP019777:3917363-3917596 [Methylomonas koyamae]
MATSTVFTNNRSQAVRLPADMRFPDSVKKVEVRAVGQERIIAPADAAWDSFFLDGPSVSDDFLNERPSQQQGERESF

Nucleotide

• Length: 234 bp

>AT33045 NZ_AP019777:3917363-3917596 [Methylomonas koyamae]
ATGGCGACCAGTACCGTATTTACCAACAACCGCAGCCAAGCCGTGCGCCTGCCCGCCGACATGCGTTTTCCCGACAGCGT
CAAAAAGGTTGAGGTTCGTGCCGTCGGGCAGGAACGCATCATTGCCCCGGCGGACGCGGCGTGGGACAGCTTTTTTCTCG
ATGGCCCGAGCGTCAGCGACGATTTTTTAAACGAACGCCCGAGCCAGCAGCAAGGCGAGCGCGAGAGTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A291IN90