Detailed information of TA system TA033044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 3440524..3441032
• Replicon: chromosome
• Accession: NZ_AP019777
• Organism: Methylomonas koyamae strain Fw12E-Y

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MKFW12EY_RS15425
• Protein ID: WP_054761864.1
• Coordinates: 3440524..3440784 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A172U6C3
• Locus tag: MKFW12EY_RS15430
• Protein ID: WP_054761862.1
• Coordinates: 3440781..3441032 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKFW12EY_RS15400 (MKFW12EY_30740)	3436364..3437488	+	1125	WP_221053314.1	urea ABC transporter permease subunit UrtC	-
MKFW12EY_RS15405 (MKFW12EY_30750)	3437485..3438294	+	810	WP_221053315.1	urea ABC transporter ATP-binding protein UrtD	-
MKFW12EY_RS15410 (MKFW12EY_30760)	3438294..3439067	+	774	WP_054761870.1	ABC transporter ATP-binding protein	-
MKFW12EY_RS15415 (MKFW12EY_30770)	3439184..3439489	+	306	WP_157199387.1	hypothetical protein	-
MKFW12EY_RS15420 (MKFW12EY_30780)	3439502..3440353	+	852	WP_054761866.1	urease accessory protein UreD	-
MKFW12EY_RS15425 (MKFW12EY_30790)	3440524..3440784	-	261	WP_054761864.1	Txe/YoeB family addiction module toxin	Toxin
MKFW12EY_RS15430 (MKFW12EY_30800)	3440781..3441032	-	252	WP_054761862.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MKFW12EY_RS15435 (MKFW12EY_30810)	3441166..3441468	+	303	WP_054761860.1	urease subunit gamma	-
MKFW12EY_RS15440 (MKFW12EY_30820)	3441599..3441916	+	318	WP_054761858.1	urease subunit beta	-
MKFW12EY_RS15445 (MKFW12EY_30830)	3441913..3443616	+	1704	WP_221053316.1	urease subunit alpha	-
MKFW12EY_RS15450 (MKFW12EY_30840)	3443859..3444290	+	432	WP_221053317.1	urease accessory protein UreE	-
MKFW12EY_RS15455 (MKFW12EY_30850)	3444280..3444954	+	675	WP_054761880.1	urease accessory protein UreF	-
MKFW12EY_RS15460 (MKFW12EY_30860)	3445215..3445832	+	618	WP_054761856.1	urease accessory protein UreG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10343.85 Da
• Isoelectric Point: 9.5752

>T33044 WP_054761864.1 NZ_AP019777:c3440784-3440524 [Methylomonas koyamae]
VKITFSSDAWDSYLYWQATDKAILKRINQLIKEIQRQPFEGIGKPEPLKHGLSGYWSRRINDEHRIVYKVAGDMLWIAQL
RYHYEY

Nucleotide

• Length: 261 bp

>T33044 NZ_AP019777:c3440784-3440524 [Methylomonas koyamae]
GTGAAAATCACCTTTTCGTCCGACGCCTGGGACAGTTACCTGTACTGGCAAGCCACCGACAAAGCCATACTTAAACGCAT
TAACCAACTGATAAAGGAAATTCAGCGCCAACCCTTCGAAGGCATCGGCAAGCCGGAACCCCTGAAACACGGTTTGTCCG
GCTATTGGTCGCGGCGGATTAACGACGAGCATAGGATCGTTTACAAGGTTGCCGGCGATATGTTGTGGATTGCTCAGTTG
CGGTATCACTATGAGTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9170.43 Da
• Isoelectric Point: 4.4621

>AT33044 WP_054761862.1 NZ_AP019777:c3441032-3440781 [Methylomonas koyamae]
MDAISYSAARANLAKTMDKVCADHAPIIITRKSESPVVMLSLEDYQAMEETAYLLRSPANARRLLESIAELESGQGSERE
LIE

Nucleotide

• Length: 252 bp

>AT33044 NZ_AP019777:c3441032-3440781 [Methylomonas koyamae]
ATGGACGCCATCAGTTACAGCGCCGCTCGCGCCAATTTAGCCAAAACCATGGACAAGGTTTGTGCCGACCATGCCCCCAT
CATCATCACCCGCAAAAGCGAGTCGCCGGTGGTGATGCTGTCGCTGGAAGACTACCAAGCCATGGAAGAAACCGCCTACC
TGCTGCGCTCGCCAGCCAACGCCCGCCGCTTGCTGGAATCCATTGCCGAATTGGAATCCGGCCAAGGCAGCGAGCGCGAG
TTGATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A172U6C3