Detailed information of TA system TA033041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1306076..1306611
• Replicon: chromosome
• Accession: NZ_AP019777
• Organism: Methylomonas koyamae strain Fw12E-Y

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKFW12EY_RS06350
• Protein ID: WP_221054204.1
• Coordinates: 1306076..1306399 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MKFW12EY_RS06355
• Protein ID: WP_197495868.1
• Coordinates: 1306396..1306611 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKFW12EY_RS06340 (MKFW12EY_12530)	1303909..1304658	+	750	WP_054763859.1	IS5 family transposase	-
MKFW12EY_RS06345 (MKFW12EY_12540)	1304706..1305692	-	987	WP_245006449.1	IS110 family transposase	-
MKFW12EY_RS06350 (MKFW12EY_12550)	1306076..1306399	-	324	WP_221054204.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKFW12EY_RS06355 (MKFW12EY_12560)	1306396..1306611	-	216	WP_197495868.1	antitoxin MazE family protein	Antitoxin
MKFW12EY_RS06360 (MKFW12EY_12570)	1306935..1307371	-	437	Protein_1261	transposase	-
MKFW12EY_RS06365 (MKFW12EY_12580)	1307714..1308508	-	795	WP_221054205.1	hypothetical protein	-
MKFW12EY_RS06370 (MKFW12EY_12590)	1308692..1309789	+	1098	WP_221053058.1	IS5 family transposase	-
MKFW12EY_RS06375 (MKFW12EY_12610)	1310579..1311063	+	485	Protein_1264	transposase	-
MKFW12EY_RS06380 (MKFW12EY_12620)	1311204..1311557	-	354	WP_221054206.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1303909..1304658	749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (8-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11410.25 Da
• Isoelectric Point: 7.2037

>T33041 WP_221054204.1 NZ_AP019777:c1306399-1306076 [Methylomonas koyamae]
MRRGDLVTVALQGDLGKPRPALLIQSDLFDAHPSVTILPVTGELRDAPLFRIPLAANEQTGLSKPSQVMVDKPQSVSRSK
IGAVIGHVDDETLLAVNRALAVFLGFA

Nucleotide

• Length: 324 bp

>T33041 NZ_AP019777:c1306399-1306076 [Methylomonas koyamae]
ATGAGGCGCGGCGATCTAGTGACGGTCGCGTTACAGGGCGATCTTGGCAAACCCAGACCGGCGCTGCTGATTCAGTCCGA
TTTGTTCGACGCGCACCCATCCGTGACGATTTTGCCGGTTACCGGCGAGTTACGCGATGCCCCGTTGTTTCGAATCCCCT
TGGCGGCCAATGAACAAACCGGGCTTAGCAAGCCTTCGCAAGTGATGGTGGATAAGCCGCAATCCGTGTCGCGCAGTAAA
ATCGGCGCCGTTATCGGACATGTGGATGACGAAACCCTGTTGGCGGTCAACCGAGCATTGGCGGTATTTTTGGGGTTTGC
CTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8254.28 Da
• Isoelectric Point: 6.4478

>AT33041 WP_197495868.1 NZ_AP019777:c1306611-1306396 [Methylomonas koyamae]
MMSISTSARVQKHRQSLREAGLRPLQIWVPDTRRNGFADECRRQSLLIRDDAADQETCMWLEAVADHDGWQ

Nucleotide

• Length: 216 bp

>AT33041 NZ_AP019777:c1306611-1306396 [Methylomonas koyamae]
ATGATGAGTATCAGTACTTCGGCACGTGTGCAAAAACATCGCCAGTCATTAAGGGAGGCGGGGTTGCGCCCCCTGCAAAT
TTGGGTGCCCGATACCCGGCGTAATGGTTTTGCCGACGAATGCCGGCGGCAATCCTTGCTGATTCGTGATGATGCCGCGG
ATCAGGAGACTTGTATGTGGCTGGAAGCGGTGGCTGACCACGACGGCTGGCAATGA