Detailed information of TA system TA033040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-AbrB
• Location: 1351006..1351652
• Replicon: chromosome
• Accession: NZ_AP019770
• Organism: Metallosphaera sedula strain MJ1HA

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A088E7Y6
• Locus tag: M2Y50_RS07100
• Protein ID: WP_012021252.1
• Coordinates: 1351006..1351395 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: M2Y50_RS07105
• Protein ID: WP_012021253.1
• Coordinates: 1351392..1351652 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2Y50_RS07080 (MJ1HA_1511)	1346742..1347923	+	1182	WP_193453307.1	mandelate racemase/muconate lactonizing enzyme family protein	-
M2Y50_RS07085 (MJ1HA_1512)	1347992..1348615	-	624	WP_012021249.1	PIG-L family deacetylase	-
M2Y50_RS07090 (MJ1HA_1513)	1348650..1349693	-	1044	WP_012021250.1	HoxN/HupN/NixA family nickel/cobalt transporter	-
M2Y50_RS07095 (MJ1HA_1514)	1349786..1350949	-	1164	WP_012021251.1	glycosyltransferase	-
M2Y50_RS07100 (MJ1HA_1515)	1351006..1351395	-	390	WP_012021252.1	type II toxin-antitoxin system VapC family toxin	Toxin
M2Y50_RS07105 (MJ1HA_1516)	1351392..1351652	-	261	WP_012021253.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
M2Y50_RS07110 (MJ1HA_1517)	1351718..1352344	-	627	WP_048060318.1	type 1 glutamine amidotransferase	-
M2Y50_RS07115 (MJ1HA_1519)	1352573..1353223	+	651	WP_012021255.1	cyclase family protein	-
M2Y50_RS07120 (MJ1HA_1520)	1353225..1353734	+	510	WP_012021256.1	OsmC family protein	-
M2Y50_RS07125 (MJ1HA_1521)	1353920..1355272	+	1353	WP_248546973.1	APC family permease	-
M2Y50_RS07130 (MJ1HA_1522)	1355287..1355781	-	495	WP_248546974.1	hypothetical protein	-
M2Y50_RS07135 (MJ1HA_1523)	1356091..1356543	+	453	WP_012021259.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 15075.63 Da
• Isoelectric Point: 4.9164

>T33040 WP_012021252.1 NZ_AP019770:c1351395-1351006 [Metallosphaera sedula]
MIFLDANFLVYLNLGVDPVKSFYLRILSNESMGVDPLVLDEVIYVSRKKYGVNFGDTLRFLDELVLPYVVVFPISLREYQ
VSKELITRYSLYPSDAFHVAVMLNNSIKKILTEDSDFEKIKEIERIWIK

Nucleotide

• Length: 390 bp

>T33040 NZ_AP019770:c1351395-1351006 [Metallosphaera sedula]
TTGATATTCTTGGATGCAAACTTCTTGGTGTACCTAAACCTGGGAGTTGACCCGGTCAAGAGCTTCTACCTCAGGATACT
TTCCAATGAGAGTATGGGCGTTGACCCCCTGGTCCTAGACGAGGTGATCTACGTCTCGAGGAAGAAGTATGGCGTGAATT
TCGGCGACACATTGCGATTTCTGGACGAGCTAGTCTTACCTTACGTAGTGGTATTTCCGATCTCCCTGAGGGAGTACCAG
GTTAGCAAGGAGCTCATCACTAGGTATTCCCTCTATCCCTCAGACGCGTTTCACGTGGCAGTAATGCTCAACAACTCCAT
AAAGAAGATTCTCACCGAGGACTCCGACTTCGAGAAGATCAAGGAAATAGAGAGGATCTGGATAAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10073.56 Da
• Isoelectric Point: 4.8329

>AT33040 WP_012021253.1 NZ_AP019770:c1351652-1351392 [Metallosphaera sedula]
MTLRVEVGKKGYMIIPKNVRDLLGINEGDFLELRVENGKIVLEKERKVDLEEVERKFEEHERRIAYARRASLGDLKGVVL
EEEFDD

Nucleotide

• Length: 261 bp

>AT33040 NZ_AP019770:c1351652-1351392 [Metallosphaera sedula]
TTGACTCTTAGGGTAGAGGTAGGAAAGAAGGGTTACATGATAATCCCCAAGAACGTCAGAGACCTGCTCGGCATCAATGA
GGGCGACTTCCTGGAACTCAGGGTAGAGAATGGCAAGATAGTCCTGGAAAAGGAAAGGAAAGTTGATCTGGAGGAGGTGG
AGAGGAAGTTTGAGGAACACGAGAGAAGGATCGCGTATGCAAGAAGGGCCAGCCTTGGAGATTTAAAGGGCGTCGTGTTG
GAGGAGGAGTTTGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A088E7Y6