Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5015678..5016175 Replicon chromosome
Accession NZ_AP019761
Organism Escherichia coli O111:H- strain 110512

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EC110512_RS25530 Protein ID WP_001173343.1
Coordinates 5016002..5016175 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EC110512_RS25525 Protein ID WP_000398619.1
Coordinates 5015678..5015974 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC110512_RS25505 5012409..5013128 - 720 WP_000611299.1 two-component system response regulator DcuR -
EC110512_RS25510 5013125..5014756 - 1632 WP_001216487.1 sensor histidine kinase -
EC110512_RS25515 5014937..5015167 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
EC110512_RS25520 5015179..5015451 + 273 WP_000405647.1 N-acetyltransferase -
EC110512_RS25525 5015678..5015974 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EC110512_RS25530 5016002..5016175 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EC110512_RS25535 5016294..5017034 - 741 Protein_4884 lysine--tRNA ligase -
EC110512_RS25540 5017068..5018281 + 1214 WP_162829202.1 IS3 family transposase -
EC110512_RS25545 5018317..5018478 - 162 Protein_4886 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T33032 WP_001173343.1 NZ_AP019761:5016002-5016175 [Escherichia coli O111:H-]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T33032 NZ_AP019761:5016002-5016175 [Escherichia coli O111:H-]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT33032 WP_000398619.1 NZ_AP019761:5015678-5015974 [Escherichia coli O111:H-]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT33032 NZ_AP019761:5015678-5015974 [Escherichia coli O111:H-]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References