Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3550258..3550912 Replicon chromosome
Accession NZ_AP019761
Organism Escherichia coli O111:H- strain 110512

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EC110512_RS18360 Protein ID WP_000244777.1
Coordinates 3550258..3550665 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EC110512_RS18365 Protein ID WP_000354046.1
Coordinates 3550646..3550912 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC110512_RS18340 3546215..3547948 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
EC110512_RS18345 3547954..3548664 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EC110512_RS18350 3548689..3549585 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EC110512_RS18355 3549697..3550218 + 522 WP_001055874.1 flavodoxin FldB -
EC110512_RS18360 3550258..3550665 - 408 WP_000244777.1 toxin CptA Toxin
EC110512_RS18365 3550646..3550912 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EC110512_RS18370 3551155..3552135 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
EC110512_RS18375 3552331..3552990 - 660 WP_000250274.1 hemolysin III family protein -
EC110512_RS18380 3553154..3553465 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EC110512_RS18385 3553510..3554943 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
EC110512_RS18390 3555000..3555743 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T33029 WP_000244777.1 NZ_AP019761:c3550665-3550258 [Escherichia coli O111:H-]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T33029 NZ_AP019761:c3550665-3550258 [Escherichia coli O111:H-]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT33029 WP_000354046.1 NZ_AP019761:c3550912-3550646 [Escherichia coli O111:H-]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT33029 NZ_AP019761:c3550912-3550646 [Escherichia coli O111:H-]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References