Detailed information of TA system TA033026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3103327..3103952
• Replicon: chromosome
• Accession: NZ_AP019761
• Organism: Escherichia coli O111:H- strain 110512

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EC110512_RS16200
• Protein ID: WP_000911330.1
• Coordinates: 3103327..3103725 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EC110512_RS16205
• Protein ID: WP_000450524.1
• Coordinates: 3103725..3103952 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC110512_RS16180	3099205..3099405	+	201	WP_000383836.1	YpfN family protein	-
EC110512_RS16185	3099515..3100213	-	699	WP_000679812.1	esterase	-
EC110512_RS16190	3100287..3102302	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
EC110512_RS16195	3102317..3103180	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
EC110512_RS16200	3103327..3103725	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EC110512_RS16205	3103725..3103952	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EC110512_RS16210	3104106..3104819	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EC110512_RS16215	3105032..3106066	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EC110512_RS16220	3106083..3106961	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EC110512_RS16225	3107107..3107691	+	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
EC110512_RS16230	3107691..3108161	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T33026 WP_000911330.1 NZ_AP019761:c3103725-3103327 [Escherichia coli O111:H-]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T33026 NZ_AP019761:c3103725-3103327 [Escherichia coli O111:H-]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT33026 WP_000450524.1 NZ_AP019761:c3103952-3103725 [Escherichia coli O111:H-]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT33026 NZ_AP019761:c3103952-3103725 [Escherichia coli O111:H-]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33