33010

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1345768..1346239 Replicon chromosome
Accession NZ_AP019761
Organism Escherichia coli O111:H- strain 110512

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag EC110512_RS06730 Protein ID WP_001303511.1
Coordinates 1345768..1346046 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EC110512_RS06735 Protein ID WP_001302048.1
Coordinates 1346048..1346239 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC110512_RS06695 1341075..1341344 - 270 WP_000003742.1 excisionase -
EC110512_RS06700 1341406..1343871 - 2466 WP_000048442.1 exonuclease -
EC110512_RS06705 1343964..1344155 - 192 WP_001090200.1 DUF1482 family protein -
EC110512_RS06710 1344152..1344340 - 189 WP_000449192.1 cell division inhibition protein DicB -
EC110512_RS06720 1344824..1345042 - 219 WP_001171930.1 hypothetical protein -
EC110512_RS06725 1345083..1345472 - 390 WP_001447517.1 hypothetical protein -
EC110512_RS06730 1345768..1346046 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EC110512_RS06735 1346048..1346239 - 192 WP_001302048.1 hypothetical protein Antitoxin
EC110512_RS06740 1346260..1346631 - 372 WP_001169687.1 hypothetical protein -
EC110512_RS06745 1346729..1347031 + 303 WP_001444689.1 transcriptional regulator -
EC110512_RS06750 1347028..1347453 + 426 WP_000693943.1 Rha family transcriptional regulator -
EC110512_RS06755 1347476..1348210 + 735 Protein_1284 helix-turn-helix domain-containing protein -
EC110512_RS06760 1348161..1348331 + 171 WP_146868674.1 type I toxin-antitoxin system Hok family toxin -
EC110512_RS06770 1348499..1348771 + 273 WP_012817871.1 hypothetical protein -
EC110512_RS06775 1348773..1349828 + 1056 WP_001369253.1 DUF968 domain-containing protein -
EC110512_RS06780 1349829..1350194 + 366 WP_000140024.1 RusA family crossover junction endodeoxyribonuclease -
EC110512_RS06785 1350203..1350733 + 531 WP_000640048.1 DUF1133 family protein -
EC110512_RS06790 1350975..1351172 + 198 WP_000917750.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espX7/nleL / espN / espK 1332819..1392054 59235


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T33010 WP_001303511.1 NZ_AP019761:c1346046-1345768 [Escherichia coli O111:H-]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T33010 NZ_AP019761:c1346046-1345768 [Escherichia coli O111:H-]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT33010 WP_001302048.1 NZ_AP019761:c1346239-1346048 [Escherichia coli O111:H-]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT33010 NZ_AP019761:c1346239-1346048 [Escherichia coli O111:H-]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
AlphaFold DB A0A829CTP0

References