Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 521973..522591 Replicon chromosome
Accession NZ_AP019761
Organism Escherichia coli O111:H- strain 110512

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EC110512_RS02470 Protein ID WP_001291435.1
Coordinates 521973..522191 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EC110512_RS02475 Protein ID WP_000344800.1
Coordinates 522217..522591 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC110512_RS02435 517262..517834 + 573 WP_000779826.1 lipoprotein -
EC110512_RS02440 517865..518176 - 312 WP_000409911.1 MGMT family protein -
EC110512_RS02450 518555..518908 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EC110512_RS02455 518950..520500 - 1551 WP_001369170.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EC110512_RS02460 520664..521134 - 471 WP_001345013.1 YlaC family protein -
EC110512_RS02465 521250..521801 - 552 WP_000102564.1 maltose O-acetyltransferase -
EC110512_RS02470 521973..522191 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EC110512_RS02475 522217..522591 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EC110512_RS02480 523137..526286 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
EC110512_RS02485 526309..527502 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33008 WP_001291435.1 NZ_AP019761:c522191-521973 [Escherichia coli O111:H-]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T33008 NZ_AP019761:c522191-521973 [Escherichia coli O111:H-]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT33008 WP_000344800.1 NZ_AP019761:c522591-522217 [Escherichia coli O111:H-]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT33008 NZ_AP019761:c522591-522217 [Escherichia coli O111:H-]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References