Detailed information of TA system TA033007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 487677..488514
• Replicon: chromosome
• Accession: NZ_AP019761
• Organism: Escherichia coli O111:H- strain 110512

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: EC110512_RS02300
• Protein ID: WP_000227784.1
• Coordinates: 487677..488219 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EC110512_RS02305
• Protein ID: WP_001297137.1
• Coordinates: 488203..488514 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC110512_RS02280	483216..484127	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
EC110512_RS02285	484295..484786	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
EC110512_RS02290	484914..486278	-	1365	WP_001000988.1	MFS transporter	-
EC110512_RS02295	486686..487621	+	936	WP_001345016.1	sel1 repeat family protein	-
EC110512_RS02300	487677..488219	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
EC110512_RS02305	488203..488514	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EC110512_RS02310	488699..489589	-	891	WP_000971336.1	heme o synthase	-
EC110512_RS02315	489601..489930	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EC110512_RS02320	489930..490544	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EC110512_RS02325	490534..492525	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EC110512_RS02330	492547..493494	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T33007 WP_000227784.1 NZ_AP019761:c488219-487677 [Escherichia coli O111:H-]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T33007 NZ_AP019761:c488219-487677 [Escherichia coli O111:H-]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT33007 WP_001297137.1 NZ_AP019761:c488514-488203 [Escherichia coli O111:H-]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT33007 NZ_AP019761:c488514-488203 [Escherichia coli O111:H-]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3