Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3362822..3363439 Replicon chromosome
Accession NZ_AP019753
Organism Pantoea ananatis strain FU-01

Toxin (Protein)


Gene name Hha Uniprot ID D4GLR0
Locus tag PAFU01_RS15875 Protein ID WP_013024903.1
Coordinates 3363224..3363439 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A8A4KA49
Locus tag PAFU01_RS15870 Protein ID WP_015700827.1
Coordinates 3362822..3363199 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAFU01_RS15840 (PAFU01_30940) 3359106..3359360 + 255 WP_014606427.1 type B 50S ribosomal protein L31 -
PAFU01_RS15845 (PAFU01_30950) 3359372..3359512 + 141 WP_014606428.1 type B 50S ribosomal protein L36 -
PAFU01_RS15850 (PAFU01_30960) 3359572..3360450 - 879 WP_019106635.1 metal ABC transporter substrate-binding protein -
PAFU01_RS15855 (PAFU01_30970) 3360463..3361302 - 840 WP_013024907.1 metal ABC transporter permease -
PAFU01_RS15860 (PAFU01_30980) 3361299..3361967 - 669 WP_014606429.1 ABC transporter ATP-binding protein -
PAFU01_RS15865 (PAFU01_30990) 3362322..3362675 + 354 WP_013024905.1 hypothetical protein -
PAFU01_RS15870 (PAFU01_31000) 3362822..3363199 + 378 WP_015700827.1 Hha toxicity modulator TomB Antitoxin
PAFU01_RS15875 (PAFU01_31010) 3363224..3363439 + 216 WP_013024903.1 HHA domain-containing protein Toxin
PAFU01_RS15885 (PAFU01_31020) 3363837..3364148 + 312 WP_014606432.1 MGMT family protein -
PAFU01_RS15890 (PAFU01_31030) 3364197..3364754 - 558 WP_019106636.1 YbaY family lipoprotein -
PAFU01_RS15895 (PAFU01_31040) 3364947..3365810 + 864 WP_116197519.1 acyl-CoA thioesterase II -
PAFU01_RS15900 (PAFU01_31050) 3365857..3367146 - 1290 WP_014606435.1 ammonium transporter AmtB -
PAFU01_RS15905 (PAFU01_31060) 3367181..3367519 - 339 WP_013024898.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8552.98 Da        Isoelectric Point: 9.4825

>T32998 WP_013024903.1 NZ_AP019753:3363224-3363439 [Pantoea ananatis]
MNKTLTKTDYLMRLRRCRSIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKVPVSVWKFVR

Download         Length: 216 bp

>T32998 NZ_AP019753:3363224-3363439 [Pantoea ananatis]
ATGAACAAAACCCTAACTAAAACTGATTATTTGATGCGACTGAGGCGCTGTCGTTCTATTGACACGCTGGAACGCGTGAT
TGAAAAAAATAAGTACGAATTACCCGATGAAGAATTAGCAGTATTTTACTCAGCAGCCGATCACCGCCTGGCTGAACTGA
CGATGAACAAGCTCTATGACAAAGTCCCGGTGTCAGTCTGGAAATTTGTACGCTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14722.35 Da        Isoelectric Point: 4.3976

>AT32998 WP_015700827.1 NZ_AP019753:3362822-3363199 [Pantoea ananatis]
MDEYSPKRHDIAQLKYLCENLFDDSMATLTDSHHGWVNDPTAESNLQLNDLIEHIASFTMNYKIKHVEDEALITQIDEYL
DDTFMLFSNYSVNTQDLQRWQRSAKRLFNVFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT32998 NZ_AP019753:3362822-3363199 [Pantoea ananatis]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACTTGTGTGAGAATCTGTTTGACGACAGCATGGC
GACATTAACTGACAGTCATCACGGCTGGGTTAATGACCCCACTGCCGAAAGCAATTTACAGCTAAACGATTTGATTGAGC
ACATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGACGAAGCACTGATTACCCAAATCGATGAATATCTT
GATGATACCTTTATGCTGTTTAGCAACTACAGTGTAAATACTCAGGATCTTCAGCGCTGGCAACGCTCAGCGAAACGTTT
ATTTAATGTGTTTACTGAAGAGTGCGCTTTTCTTCAACAGCCAAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References