Detailed information of TA system TA032979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2234693..2235222
• Replicon: chromosome
• Accession: NZ_AP019751
• Organism: Staphylococcus aureus strain JRA307

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FPG46_RS11495
• Protein ID: WP_000621175.1
• Coordinates: 2234693..2235055 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FPG46_RS11500
• Protein ID: WP_000948331.1
• Coordinates: 2235052..2235222 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPG46_RS11475	2231671..2232441	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
FPG46_RS11480	2232416..2232895	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
FPG46_RS11485	2232897..2233223	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FPG46_RS11490	2233342..2234343	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FPG46_RS11495	2234693..2235055	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPG46_RS11500	2235052..2235222	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FPG46_RS11505	2235307..2236455	-	1149	WP_001281154.1	alanine racemase	-
FPG46_RS11510	2236521..2236880	-	360	WP_000581197.1	holo-ACP synthase	-
FPG46_RS11515	2236884..2237375	-	492	WP_001205912.1	PH domain-containing protein	-
FPG46_RS11520	2237362..2238945	-	1584	WP_144079000.1	PH domain-containing protein	-
FPG46_RS11525	2238938..2239417	-	480	WP_001287078.1	hypothetical protein	-
FPG46_RS11530	2239626..2240186	-	561	WP_144079001.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T32979 WP_000621175.1 NZ_AP019751:c2235055-2234693 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T32979 NZ_AP019751:c2235055-2234693 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT32979 WP_000948331.1 NZ_AP019751:c2235222-2235052 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT32979 NZ_AP019751:c2235222-2235052 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1