Detailed information of TA system TA032929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518253..518889
• Replicon: chromosome
• Accession: NZ_AP019714
• Organism: Bacillus subtilis subsp. subtilis strain NBRC 13719

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FNP71_RS02675
• Protein ID: WP_003156187.1
• Coordinates: 518539..518889 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: FNP71_RS02670
• Protein ID: WP_003225183.1
• Coordinates: 518253..518534 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP71_RS02650	514612..515211	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
FNP71_RS02655	515306..515671	+	366	WP_003234281.1	holo-ACP synthase	-
FNP71_RS02660	515837..516853	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
FNP71_RS02665	516968..518137	+	1170	WP_003234284.1	alanine racemase	-
FNP71_RS02670	518253..518534	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FNP71_RS02675	518539..518889	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FNP71_RS02680	519004..519828	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
FNP71_RS02685	519833..520198	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
FNP71_RS02690	520202..520603	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
FNP71_RS02695	520615..521622	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
FNP71_RS02700	521684..522013	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
FNP71_RS02705	522010..522492	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
FNP71_RS02710	522458..523246	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
FNP71_RS02715	523246..523845	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T32929 WP_003156187.1 NZ_AP019714:518539-518889 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T32929 NZ_AP019714:518539-518889 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT32929 WP_003225183.1 NZ_AP019714:518253-518534 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT32929 NZ_AP019714:518253-518534 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32