Detailed information of TA system TA032923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102097..2102626
• Replicon: chromosome
• Accession: NZ_AP019712
• Organism: Staphylococcus aureus strain Tokyo12480

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GOZ34_RS10325
• Protein ID: WP_000621175.1
• Coordinates: 2102097..2102459 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GOZ34_RS10330
• Protein ID: WP_000948331.1
• Coordinates: 2102456..2102626 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOZ34_RS10305	2099076..2099846	-	771	WP_155978257.1	RNA polymerase sigma factor SigB	-
GOZ34_RS10310	2099821..2100300	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
GOZ34_RS10315	2100302..2100628	-	327	WP_001052491.1	anti-sigma factor antagonist	-
GOZ34_RS10320	2100747..2101748	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GOZ34_RS10325	2102097..2102459	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GOZ34_RS10330	2102456..2102626	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GOZ34_RS10335	2102711..2103859	-	1149	WP_072512344.1	alanine racemase	-
GOZ34_RS10340	2103925..2104284	-	360	WP_029550315.1	holo-ACP synthase	-
GOZ34_RS10345	2104288..2104779	-	492	WP_072435149.1	PH domain-containing protein	-
GOZ34_RS10350	2104772..2106349	-	1578	WP_155978258.1	PH domain-containing protein	-
GOZ34_RS10355	2106342..2106821	-	480	WP_089535597.1	hypothetical protein	-
GOZ34_RS10360	2107030..2107590	-	561	WP_070974392.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T32923 WP_000621175.1 NZ_AP019712:c2102459-2102097 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T32923 NZ_AP019712:c2102459-2102097 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT32923 WP_000948331.1 NZ_AP019712:c2102626-2102456 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT32923 NZ_AP019712:c2102626-2102456 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1